{"doi":"10.1371/journal.pone.0232757","title":"Characterization of a human monoclonal antibody generated from a B-cell specific for a prefusion-stabilized spike protein of Middle East respiratory syndrome coronavirus","abstract":"Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory infection and continues to infect humans, thereby contributing to a high mortality rate (34.3% in 2019). In the absence of an available licensed vaccine and antiviral agent, therapeutic human antibodies have been suggested as candidates for treatment. In this study, human monoclonal antibodies were isolated by sorting B cells from patient's PBMC cells with prefusion stabilized spike (S) probes and a direct immunoglobulin cloning strategy. We identified six receptor-binding domain (RBD)-specific and five S1 (non-RBD)-specific antibodies, among which, only the RBD-specific antibodies showed high neutralizing potency (IC50 0.006-1.787 μg/ml) as well as high affinity to RBD. Notably, passive immunization using a highly potent antibody (KNIH90-F1) at a relatively low dose (2 mg/kg) completely protected transgenic mice expressing human DPP4 against MERS-CoV lethal challenge. These results suggested that human monoclonal antibodies isolated by using the rationally designed prefusion MERS-CoV S probe could be considered potential candidates for the development of therapeutic and/or prophylactic antiviral agents for MERS-CoV human infection.","journal":"PLoS ONE","year":2020,"id":79410,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9567,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":413019,"name":"Hye-Min Woo","orcid":null,"position":1,"is_corresponding":false},{"id":413020,"name":"Tae-Young Lee","orcid":null,"position":2,"is_corresponding":false},{"id":412302,"name":"So Young Lee","orcid":"0000-0003-3130-785X","position":3,"is_corresponding":false},{"id":413021,"name":"Sang‐Mu Shim","orcid":null,"position":4,"is_corresponding":false},{"id":412303,"name":"Woo Jung Park","orcid":"0000-0001-9804-3444","position":5,"is_corresponding":false},{"id":412304,"name":"Jeong‐Sun Yang","orcid":"0000-0001-5005-9738","position":6,"is_corresponding":false},{"id":413022,"name":"Joo Ae Kim","orcid":null,"position":7,"is_corresponding":false},{"id":413023,"name":"Miran Yun","orcid":null,"position":8,"is_corresponding":false},{"id":115303,"name":"Dae-Won Kim","orcid":null,"position":9,"is_corresponding":false},{"id":36864,"name":"Sung Soon Kim","orcid":"0000-0003-2774-6710","position":10,"is_corresponding":false},{"id":105589,"name":"Yi Zhang","orcid":"0000-0002-8228-2560","position":11,"is_corresponding":false},{"id":105599,"name":"Wei Shi","orcid":"0000-0002-4770-6444","position":12,"is_corresponding":false},{"id":105588,"name":"Lingshu Wang","orcid":"0000-0002-8404-4910","position":13,"is_corresponding":false},{"id":87311,"name":"Barney S. Graham","orcid":"0000-0001-8112-0853","position":14,"is_corresponding":false},{"id":105614,"name":"John R. Mascola","orcid":"0000-0002-5293-4695","position":15,"is_corresponding":false},{"id":413024,"name":"Nanshuang Wang","orcid":null,"position":16,"is_corresponding":false},{"id":87312,"name":"Jason S. McLellan","orcid":"0000-0003-3991-542X","position":17,"is_corresponding":false},{"id":412305,"name":"Joo-Yeon Lee","orcid":"0000-0002-0993-9626","position":18,"is_corresponding":false},{"id":412306,"name":"Hansaem Lee","orcid":"0000-0002-9430-8123","position":19,"is_corresponding":false},{"id":412301,"name":"Jang-Hoon Choi","orcid":"0000-0002-7734-478X","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":null,"created_at":"2026-07-18T21:50:33.748601Z","pmid":"32384116","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}