{"doi":"10.1371/journal.pone.0227114","title":"Discovery of Jogalong virus, a novel hepacivirus identified in a Culex annulirostris (Skuse) mosquito from the Kimberley region of Western Australia","abstract":"The discovery of hepaciviruses in non-human hosts has accelerated following the advancement of high-throughput sequencing technology. Hepaciviruses have now been described in reptiles, fish, birds, and an extensive array of mammals. Using metagenomic sequencing on pooled samples of field-collected Culex annulirostris mosquitoes, we discovered a divergent hepacivirus-like sequence, named Jogalong virus, from the Kimberley region in northern Western Australia. Using PCR, we screened the same 300 individual mosquitoes and found just a single positive sample (1/300, 0.33%). Phylogenetic analysis of the hepacivirus NS5B protein places Jogalong virus within the genus Hepacivirus but on a distinct and deeply rooted monophyletic branch shared with duck hepacivirus, suggesting a notably different evolutionary history. Vertebrate barcoding PCR targeting two mitochondrial genes, cytochrome c oxidase subunit I and cytochrome b, indicated that the Jogalong virus-positive mosquito had recently fed on the tawny frogmouth (Podargus strigoides), although it is currently unknown whether this bird species contributes to the natural ecology of this virus.","journal":"PLoS ONE","year":2020,"id":79165,"datarank":0.42498200160843247,"base_score":2.833213344056216,"endowment":2.833213344056216,"self_citation_contribution":0.42498200160843247,"citation_network_contribution":0.0,"self_endowment_contribution":0.42498200160843247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9415,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":411522,"name":"Avram Levy","orcid":"0000-0002-0934-2387","position":1,"is_corresponding":false},{"id":411523,"name":"Rachel A. Yates","orcid":"0000-0002-0606-1395","position":2,"is_corresponding":false},{"id":412709,"name":"Nilusha Somaweera","orcid":null,"position":3,"is_corresponding":false},{"id":411524,"name":"Peter J. Neville","orcid":"0000-0003-0823-6934","position":4,"is_corresponding":false},{"id":412710,"name":"Jay Nicholson","orcid":null,"position":5,"is_corresponding":false},{"id":412711,"name":"M.D. Lindsay","orcid":null,"position":6,"is_corresponding":false},{"id":411525,"name":"J. S. Mackenzie","orcid":"0000-0002-5918-2190","position":7,"is_corresponding":false},{"id":411526,"name":"Komal Jain","orcid":"0000-0001-7479-944X","position":8,"is_corresponding":false},{"id":411527,"name":"Allison Imrie","orcid":"0000-0003-4715-6662","position":9,"is_corresponding":false},{"id":411528,"name":"David W. Smith","orcid":"0000-0002-3107-5182","position":10,"is_corresponding":false},{"id":104439,"name":"W. Ian Lipkin","orcid":"0000-0002-8768-9386","position":11,"is_corresponding":false},{"id":72263,"name":"Simon H. Williams","orcid":"0000-0002-6276-4685","position":0,"is_corresponding":true}],"reference_count":34,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T21:50:33.748601Z","pmid":"31899786","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}