{"doi":"10.1371/journal.pone.0203415","title":"Impact of two neighbouring ribosomal protein clusters on biogenesis factor binding and assembly of yeast late small ribosomal subunit precursors","abstract":null,"journal":"PLOS ONE","year":2019,"id":673669,"datarank":0.41588830833596724,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.0,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":769919,"name":"Gisela Pöll","orcid":null,"position":1,"is_corresponding":false},{"id":1760094,"name":"Steffen Jakob","orcid":null,"position":2,"is_corresponding":false},{"id":345482,"name":"Sébastien Ferreira-Cerca","orcid":"0000-0002-0522-843X","position":3,"is_corresponding":false},{"id":769433,"name":"Joachim Griesenbeck","orcid":"0000-0002-7817-6095","position":4,"is_corresponding":false},{"id":213525,"name":"Herbert Tschochner","orcid":null,"position":5,"is_corresponding":false},{"id":769435,"name":"Philipp Milkereit","orcid":"0000-0001-8850-9140","position":6,"is_corresponding":false},{"id":1760093,"name":"Jan Linnemann","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Impact of two neighbouring ribosomal protein clusters on biogenesis factor binding and assembly of yeast late small ribosomal subunit precursors","abstract":"Many of the small ribosomal subunit proteins are required for the stabilisation of late small ribosomal subunit (SSU) precursors and for final SSU rRNA processing in S. cerevisiae. Among them are ribosomal proteins (r-proteins) which form a protein cluster around rpS0 (uS2) at the \"neck\" of the SSU (S0-cluster) and others forming a nearby protein cluster around rpS3 (uS3) at the SSU \"beak\". Here we applied semi-quantitative proteomics together with complementary biochemical approaches to study how incomplete assembly of these two r-protein clusters affects binding and release of SSU maturation factors and assembly of other r-proteins in late SSU precursors in S. cerevisiae. For each of the two clusters specific impairment of the local r-protein assembly state was observed in Rio2 associated SSU precursors. Besides, cluster-specific effects on the association of biogenesis factors were detected. These suggested a role of S0-cluster formation for the efficient release of the two nuclear export factors Rrp12 and Slx9 from SSU precursors and for the correct incorporation of the late acting biogenesis factor Rio2. Based on our and on previous results we propose the existence of at least two different r-protein assembly checkpoints during late SSU maturation in S. cerevisiae. We discuss in the light of recent SSU precursor structure models how r-protein assembly states might be sensed by biogenesis factors at the S0-cluster checkpoint.","is_dataset_classified":null,"base_score":2.772588722239781,"endowment":2.772588722239781,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30653518","pmcid":null,"openalex_id":"https://openalex.org/W2950452709","authors":[],"funders":[{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"SFB 960","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"185476941/SFB 960","title":"RNP biogenesis: assembly of ribosomes and non-ribosomal RNPs and control of their function"}],"total_grants":3,"fwci":0.5309,"citation_percentile":0.62570726,"influential_citations":0,"citation_trend":[{"year":2019,"count":2},{"year":2021,"count":3},{"year":2022,"count":2},{"year":2023,"count":6},{"year":2024,"count":1},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0203415&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0203415&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0203415","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.pone.0203415","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30653518","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6336269","host_type":"repository"},{"url":"https://hal.science/hal-04431371","host_type":"repository"},{"url":"https://doaj.org/article/f4fff90ab1e7462795ae07e097177ac5","host_type":"repository"},{"url":"https://figshare.com/articles/dataset/Impact_of_two_neighbouring_ribosomal_protein_clusters_on_biogenesis_factor_binding_and_assembly_of_yeast_late_small_ribosomal_subunit_precursors/7600874","host_type":"repository"},{"url":"https://doi.org/10.1101/398149","host_type":""},{"url":"https://dx.doi.org/10.5283/epub.38370","host_type":""},{"url":"http://dx.doi.org/10.1371/journal.pone.0203415","host_type":""},{"url":"https://hal.science/hal-04431371v1","host_type":""},{"url":"https://hal.science/hal-04431371v1/document","host_type":""},{"url":"https://dx.doi.org/10.1371/journal.pone.0203415","host_type":""},{"url":"https://dx.doi.org/10.1101/398149","host_type":""},{"url":"https://epub.uni-regensburg.de/38370/","host_type":""},{"url":"http://dx.doi.org/10.1101/398149","host_type":""}],"fields_of_study":["RNA modifications and cancer","RNA and protein synthesis mechanisms","RNA Research and Splicing","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Nuclear Proteins","Ribosomal Proteins","RNA Processing, Post-Transcriptional","RNA, Fungal","RNA, Ribosomal","Saccharomyces cerevisiae","Protein Serine-Threonine Kinases","Saccharomyces cerevisiae Proteins","Ribosome Subunits, Small, Eukaryotic"],"keywords":["Biogenesis","Ribosomal protein","Protein subunit","Ribosomal RNA","Biology","Eukaryotic Large Ribosomal Subunit","Eukaryotic Small Ribosomal Subunit","Ribosome biogenesis","Proteomics","Ribosome","Biochemistry","RNA","Gene","18S ribosomal RNA","Ribosomal Proteins","Ribosome Subunits, Small, Eukaryotic","Saccharomyces cerevisiae Proteins","Science","Q","R","Nuclear Proteins","RNA, Fungal","Saccharomyces cerevisiae","Protein Serine-Threonine Kinases","[SDV] Life Sciences [q-bio]","RNA, Ribosomal","Medicine","570 Biowissenschaften, Biologie","ddc:570","RNA Processing, Post-Transcriptional","Research Article"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T14:09:33.696754Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}