{"doi":"10.1371/journal.pone.0193059","title":"Mesenchymal stem cell-derived exosomes have altered microRNA profiles and induce osteogenic differentiation depending on the stage of differentiation","abstract":null,"journal":"PLOS ONE","year":2018,"id":624644,"datarank":0.7789435276335317,"base_score":5.19295685089021,"endowment":5.19295685089021,"self_citation_contribution":0.7789435276335317,"citation_network_contribution":0.0,"self_endowment_contribution":0.7789435276335317,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":179,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":493848,"name":"Omar Omar","orcid":"0000-0001-7582-8337","position":1,"is_corresponding":false},{"id":1614806,"name":"Forugh Vazirisani","orcid":null,"position":2,"is_corresponding":false},{"id":1614807,"name":"Peter Thomsen","orcid":null,"position":3,"is_corresponding":false},{"id":4972,"name":"Karin M. Ekström","orcid":"0000-0001-7808-4572","position":4,"is_corresponding":false},{"id":473907,"name":"Xiaoqin Wang","orcid":"0000-0003-3880-8374","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Mesenchymal stem cell-derived exosomes have altered microRNA profiles and induce osteogenic differentiation depending on the stage of differentiation","abstract":"Human mesenchymal stem cell (hMSC)-derived exosomes have shown regenerative effects, but their role in osteogenesis and the underlying mechanism are yet to be determined. In this study, we examined the time-course secretion of exosomes by hMSCs during the entire process of osteogenic differentiation. Exosomes derived from hMSCs in various stages of osteogenic differentiation committed homotypic cells to differentiate towards osteogenic lineage, but only exosomes from late stages of osteogenic differentiation induced extracellular matrix mineralisation. Exosomes from expansion and early and late stages of osteogenic differentiation were internalised by a subpopulation of hMSCs. MicroRNA profiling revealed a set of differentially expressed exosomal microRNAs from the late stage of osteogenic differentiation, which were osteogenesis related. Target prediction demonstrated that these microRNAs enriched pathways involved in regulation of osteogenic differentiation and general mechanisms how exosomes exert their functions, such as \"Wnt signalling pathway\" and \"endocytosis\". Taken together, the results show that MSCs secrete exosomes with different biological properties depending on differentiation stage of their parent cells. The exosomal cargo transferred from MSCs in the late stage of differentiation induces osteogenic differentiation and mineralisation. Moreover, it is suggested that the regulatory effect on osteogenesis by exosomes is at least partly exerted by exosomal microRNA.","is_dataset_classified":null,"base_score":5.19295685089021,"endowment":5.19295685089021,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29447276","pmcid":"PMC5814093","openalex_id":"https://openalex.org/W2785527513","authors":[],"funders":[{"funder_name":"The Swedish Research Council","grant_id":"K2015-52X-09495-28-4","title":null},{"funder_name":"LUA/ALF Research Grant","grant_id":"ALFGBG-448851","title":null},{"funder_name":"the IngaBritt and Arne Lundberg Foundation","grant_id":"2014-0049","title":null},{"funder_name":"Stiftelsen Hjalmar Svenssons Forskningsfond","grant_id":"HJSV2014073","title":null},{"funder_name":"Stiftelsen Hjalmar Svenssons Forskningsfond","grant_id":"HJSV2013030","title":null},{"funder_name":"Stiftelsen Hjalmar Svenssons Forskningsfond","grant_id":"HJSV201341","title":null},{"funder_name":"Stiftelsen Hjalmar Svenssons Forskningsfond","grant_id":"HJSV2014059","title":null},{"funder_name":"Vilhelm and Martina Lundgren Vetenskapsfond","grant_id":"107/2014","title":null},{"funder_name":"Adlerbertska Stiftelserna","grant_id":"E 2015/78","title":null},{"funder_name":"Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Area of Advance Materials of Chalmers and GU Biomaterials within the Strategic Research Area initiative launched by the Swedish Government","grant_id":"","title":null},{"funder_name":"BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy","grant_id":"","title":null},{"funder_name":"The Västra Götaland Region","grant_id":"","title":null},{"funder_name":"Doktor Felix Neuberghs stiftelse","grant_id":"","title":null}],"total_grants":14,"fwci":8.3311,"citation_percentile":0.98528533,"influential_citations":0,"citation_trend":[{"year":2018,"count":6},{"year":2019,"count":23},{"year":2020,"count":40},{"year":2021,"count":32},{"year":2022,"count":19},{"year":2023,"count":17},{"year":2024,"count":24},{"year":2025,"count":15},{"year":2026,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0193059&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0193059&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0193059","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.pone.0193059","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29447276","host_type":"repository"},{"url":"https://doaj.org/article/7d6192f9ca7e4bb0b7558fbb3f9343ec","host_type":"repository"},{"url":"https://figshare.com/articles/dataset/Mesenchymal_stem_cell-derived_exosomes_have_altered_microRNA_profiles_and_induce_osteogenic_differentiation_depending_on_the_stage_of_differentiation/5894908","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5814093","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5814093","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5814093?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1371/journal.pone.0193059","host_type":""},{"url":"https://dx.doi.org/10.1371/journal.pone.0193059","host_type":""},{"url":"https://publications.scilifelab.se/publication/093c9af07c42451d9a937b855507d4b4","host_type":""}],"fields_of_study":["Extracellular vesicles in disease","MicroRNA in disease regulation","Circular RNAs in diseases","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Alkaline Phosphatase","Calcium","Cell Differentiation","Cells, Cultured","Humans","Osteogenesis","Signal Transduction","Gene Expression Profiling","MicroRNAs","Microscopy, Electron, Transmission","Exosomes","Mesenchymal Stem Cells"],"keywords":["Mesenchymal stem cell","Microvesicles","Cell biology","microRNA","Cellular differentiation","Stem cell","Biology","Chemistry","Genetics","Science","Gene Expression Profiling","Q","R","Cell Differentiation","Mesenchymal Stem Cells","Alkaline Phosphatase","Exosomes","MicroRNAs","Microscopy, Electron, Transmission","Osteogenesis","Medicine","Humans","Calcium","Cells, Cultured","Research Article","Signal Transduction"],"sdg_mappings":[{"sdg_number":4,"sdg_label":"4. 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