{"doi":"10.1371/journal.pone.0171257","title":"Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity","abstract":null,"journal":"PLOS ONE","year":2017,"id":622369,"datarank":0.6995158641168101,"base_score":4.663439094112067,"endowment":4.663439094112067,"self_citation_contribution":0.6995158641168101,"citation_network_contribution":0.0,"self_endowment_contribution":0.6995158641168101,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":105,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":8,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1608018,"name":"Yingche Chen","orcid":null,"position":1,"is_corresponding":false},{"id":1177660,"name":"Jiahui Wu","orcid":"0000-0002-1812-4488","position":2,"is_corresponding":false},{"id":219930,"name":"Nathan C. Shaner","orcid":null,"position":3,"is_corresponding":false},{"id":219932,"name":"Robert E. Campbell","orcid":null,"position":4,"is_corresponding":false},{"id":456227,"name":"Yi Shen","orcid":"0000-0001-5043-5211","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity","abstract":"MCherry, the Discosoma sp. mushroom coral-derived monomeric red fluorescent protein (RFP), is a commonly used genetically encoded fluorophore for live cell fluorescence imaging. We have used a combination of protein design and directed evolution to develop mCherry variants with low cytotoxicity to Escherichia coli and altered excitation and emission profiles. These efforts ultimately led to a long Stokes shift (LSS)-mCherry variant (λex = 460 nm and λem = 610 nm) and a red-shifted (RDS)-mCherry variant (λex = 600 nm and λem = 630 nm). These new RFPs provide insight into the influence of the chromophore environment on mCherry's fluorescence properties, and may serve as templates for the future development of fluorescent probes for live cell imaging.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":8,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28241009","pmcid":"PMC5328254","openalex_id":null,"authors":[],"funders":[{"funder_name":"Natural Sciences and Engineering Research Council of Canada","grant_id":"unidentified","title":"unidentified"}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171257&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0171257","host_type":"publisher"},{"url":"https://doaj.org/article/47e71592e78f442aab02b3b89d90aabd","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5328254","host_type":"repository"},{"url":"https://figshare.com/articles/dataset/Engineering_of_mCherry_variants_with_long_Stokes_shift_red-shifted_fluorescence_and_low_cytotoxicity/4698565","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5328254","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5328254?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1371/journal.pone.0171257","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/28241009","host_type":""},{"url":"http://dx.doi.org/10.1371/journal.pone.0171257","host_type":""},{"url":"https://dx.doi.org/10.1371/journal.pone.0171257","host_type":""}],"fields_of_study":["0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Animals","Anthozoa","Escherichia coli","Luminescent Proteins","Oligonucleotides","Fluorescent Dyes","Protein Engineering","Mutagenesis, Site-Directed","Evolution, Molecular","Gene Library","Fluorescence","Models, Molecular","Red Fluorescent Protein"],"keywords":["Models, Molecular","Science","Q","R","Oligonucleotides","Anthozoa","Protein Engineering","Fluorescence","Evolution, Molecular","Luminescent Proteins","Escherichia coli","Mutagenesis, Site-Directed","Medicine","Animals","Research Article","Fluorescent Dyes","Gene Library","Red Fluorescent Protein"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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