{"doi":"10.1371/journal.pone.0160717","title":"Gateway Vectors for Simultaneous Detection of Multiple Protein−Protein Interactions in Plant Cells Using Bimolecular Fluorescence Complementation","abstract":null,"journal":"PLOS ONE","year":2016,"id":588814,"datarank":1.5442300917568634,"base_score":3.5553480614894135,"endowment":3.5553480614894135,"self_citation_contribution":0.5333022092234121,"citation_network_contribution":1.0109278825334513,"self_endowment_contribution":0.5333022092234121,"citer_contribution":1.0109278825334513,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":31,"citers_with_citation_signal":27,"citers_with_endowment":27,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1506454,"name":"Kazumasa Nito","orcid":null,"position":1,"is_corresponding":false},{"id":1506456,"name":"Kazumi Hikino","orcid":null,"position":2,"is_corresponding":false},{"id":1506457,"name":"Shino Goto-Yamada","orcid":null,"position":3,"is_corresponding":false},{"id":127742,"name":"Mikio Nishimura","orcid":null,"position":4,"is_corresponding":false},{"id":1506458,"name":"Tsuyoshi Nakagawa","orcid":"0000-0003-1967-0445","position":5,"is_corresponding":false},{"id":1506459,"name":"Shoji Mano","orcid":null,"position":6,"is_corresponding":false},{"id":1506452,"name":"Akane Kamigaki","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Gateway Vectors for Simultaneous Detection of Multiple Protein−Protein Interactions in Plant Cells Using Bimolecular Fluorescence Complementation","abstract":"Bimolecular fluorescence complementation (BiFC) is widely used to detect protein-protein interactions, because it is technically simple, convenient, and can be adapted for use with conventional fluorescence microscopy. We previously constructed enhanced yellow fluorescent protein (EYFP)-based Gateway cloning technology-compatible vectors. In the current study, we generated new Gateway cloning technology-compatible vectors to detect BiFC-based multiple protein-protein interactions using N- and C-terminal fragments of enhanced cyan fluorescent protein (ECFP), enhanced green fluorescent protein (EGFP), and monomeric red fluorescent protein (mRFP1). Using a combination of N- and C-terminal fragments from ECFP, EGFP and EYFP, we observed a shift in the emission wavelength, enabling the simultaneous detection of multiple protein-protein interactions. Moreover, we developed these vectors as binary vectors for use in Agrobacterium infiltration and for the generate transgenic plants. We verified that the binary vectors functioned well in tobacco cells. The results demonstrate that the BiFC vectors facilitate the design of various constructions and are convenient for the detection of multiple protein-protein interactions simultaneously in plant cells.","is_dataset_classified":null,"base_score":3.5553480614894135,"endowment":3.5553480614894135,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27490375","pmcid":"PMC4973907","openalex_id":"https://openalex.org/W2482688439","authors":[],"funders":[{"funder_name":"Ministry of Education, Culture, Sports, Science, and Technology","grant_id":"KAKENHI Grant-in-Aid for Scientific Research (C) (grant No. 26440157 to S.M)","title":null},{"funder_name":"Ministry of Education, Culture, Sports, Science, and Technology","grant_id":"KAKENHI Grant-in-Aid for Scientific Research in Priority Areas (grant No. 16085101 to M.N.)","title":null},{"funder_name":"Ministry of Education, Culture, Sports, Science, and Technology","grant_id":"KAKENIHI Grant-in-Aid for Scientific Research on Innovative Areas (No. 22120001 to M.N)","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"","title":null}],"total_grants":4,"fwci":1.1224,"citation_percentile":0.78758881,"influential_citations":0,"citation_trend":[{"year":2017,"count":1},{"year":2018,"count":3},{"year":2019,"count":3},{"year":2020,"count":1},{"year":2021,"count":4},{"year":2022,"count":6},{"year":2023,"count":6},{"year":2024,"count":6},{"year":2025,"count":2},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0160717&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0160717&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0160717","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.pone.0160717","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27490375","host_type":"repository"},{"url":"https://doaj.org/article/2102ceeb9c0e4d80bdc2c47787b98b83","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4973907","host_type":"repository"},{"url":"https://figshare.com/articles/dataset/Gateway_Vectors_for_Simultaneous_Detection_of_Multiple_Protein_Protein_Interactions_in_Plant_Cells_Using_Bimolecular_Fluorescence_Complementation/3871212","host_type":"repository"},{"url":"http://hdl.handle.net/2433/218697","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4973907","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4973907?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Photosynthetic Processes and Mechanisms","Transgenic Plants and Applications","Advanced Fluorescence Microscopy Techniques","Agrobacterium","Genetic Vectors","Green Fluorescent Proteins","Luminescent Proteins","Microscopy, Fluorescence","Plant Cells","Plant Leaves","Plant Proteins","Plants, Genetically Modified","Protein Interaction Maps","Nicotiana","Red Fluorescent Protein"],"mesh_terms":["Red Fluorescent Protein","Genetic Vectors","Luminescent Proteins","Microscopy, Fluorescence","Plant Proteins","Nicotiana","Plant Leaves","Plants, Genetically Modified","Green Fluorescent Proteins","Plant Cells","Agrobacterium","Protein Interaction Maps"],"keywords":["Bimolecular fluorescence complementation","Yellow fluorescent protein","Green fluorescent protein","Fluorescence","Protein–protein interaction","Cloning (programming)","Fluorescent protein","Förster resonance energy transfer","Agrobacterium","Biology","Biophysics","Chemistry","Transgene","Biochemistry","Physics","Gene","Computer science","Optics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-22T22:06:30.924042Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}