{"doi":"10.1371/journal.pone.0160641","title":"Molecular, Structural and Immunological Characterization of Der p 18, a Chitinase-Like House Dust Mite Allergen","abstract":null,"journal":"PLOS ONE","year":2016,"id":595319,"datarank":0.5606504427425053,"base_score":3.7376696182833684,"endowment":3.7376696182833684,"self_citation_contribution":0.5606504427425053,"citation_network_contribution":0.0,"self_endowment_contribution":0.5606504427425053,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":41,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1524383,"name":"Katharina Blatt","orcid":null,"position":1,"is_corresponding":false},{"id":1524384,"name":"Ursula Malkus","orcid":null,"position":2,"is_corresponding":false},{"id":800007,"name":"Christian Fercher","orcid":"0000-0002-3693-9357","position":3,"is_corresponding":false},{"id":1524385,"name":"Ines Swoboda","orcid":null,"position":4,"is_corresponding":false},{"id":1524386,"name":"Margit Focke-Tejkl","orcid":null,"position":5,"is_corresponding":false},{"id":1524387,"name":"Kuan-Wei Chen","orcid":null,"position":6,"is_corresponding":false},{"id":1524388,"name":"Susanne Seiberler","orcid":null,"position":7,"is_corresponding":false},{"id":1524389,"name":"Irene Mittermann","orcid":null,"position":8,"is_corresponding":false},{"id":1460625,"name":"Christian Lupinek","orcid":"0000-0002-8612-8245","position":9,"is_corresponding":false},{"id":1524391,"name":"Azahara Rodriguez-Dominguez","orcid":null,"position":10,"is_corresponding":false},{"id":1524393,"name":"Petra Zieglmayer","orcid":null,"position":11,"is_corresponding":false},{"id":1524395,"name":"René Zieglmayer","orcid":null,"position":12,"is_corresponding":false},{"id":79778,"name":"Walter Keller","orcid":"0000-0002-2261-958X","position":13,"is_corresponding":false},{"id":1524398,"name":"Vladislav Krzyzanek","orcid":null,"position":14,"is_corresponding":false},{"id":114968,"name":"Peter Valent","orcid":null,"position":15,"is_corresponding":false},{"id":144408,"name":"Rudolf Valenta","orcid":null,"position":16,"is_corresponding":false},{"id":310435,"name":"Susanne Vrtala","orcid":"0000-0003-4250-8243","position":17,"is_corresponding":false},{"id":1524382,"name":"Yvonne Resch","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Molecular, Structural and Immunological Characterization of Der p 18, a Chitinase-Like House Dust Mite Allergen","abstract":"BACKGROUND: The house dust mite (HDM) allergen Der p 18 belongs to the glycoside hydrolase family 18 chitinases. The relevance of Der p 18 for house dust mite allergic patients has only been partly investigated. OBJECTIVE: To perform a detailed characterization of Der p 18 on a molecular, structural and immunological level. METHODS: Der p 18 was expressed in E. coli, purified to homogeneity, tested for chitin-binding activity and its secondary structure was analyzed by circular dichroism. Der p 18-specific IgG antibodies were produced in rabbits to localize the allergen in mites using immunogold electron microscopy and to search for cross-reactive allergens in other allergen sources (i.e. mites, crustacea, mollusca and insects). IgE reactivity of rDer p 18 was tested with sera from clinically well characterized HDM-allergic patients (n = 98) and its allergenic activity was analyzed in basophil activation experiments. RESULTS: Recombinant Der p 18 was expressed and purified as a folded, biologically active protein. It shows weak chitin-binding activity and partial cross-reactivity with Der f 18 from D. farinae but not with proteins from the other tested allergen sources. The allergen was mainly localized in the peritrophic matrix of the HDM gut and to a lower extent in fecal pellets. Der p 18 reacted with IgE from 10% of mite allergic patients from Austria and showed allergenic activity when tested for basophil activation in Der p 18-sensitized patients. CONCLUSION: Der p 18 is a rather genus-specific minor allergen with weak chitin-binding activity but exhibits allergenic activity and therefore should be included in diagnostic test panels for HDM allergy.","is_dataset_classified":null,"base_score":3.7376696182833684,"endowment":3.7376696182833684,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27548813","pmcid":"PMC4993390","openalex_id":"https://openalex.org/W2514609206","authors":[],"funders":[],"total_grants":0,"fwci":2.2212,"citation_percentile":0.88027784,"influential_citations":0,"citation_trend":[{"year":2016,"count":1},{"year":2017,"count":5},{"year":2018,"count":4},{"year":2019,"count":3},{"year":2020,"count":5},{"year":2021,"count":5},{"year":2022,"count":3},{"year":2023,"count":6},{"year":2024,"count":3},{"year":2025,"count":4},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0160641&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0160641&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0160641","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.pone.0160641","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27548813","host_type":"repository"},{"url":"http://hdl.handle.net/11104/0263766","host_type":"repository"},{"url":"https://doaj.org/article/461a488a873d4b949bd136a203c70cca","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4993390","host_type":"repository"},{"url":"https://figshare.com/articles/dataset/Molecular_Structural_and_Immunological_Characterization_of_Der_p_18_a_Chitinase-Like_House_Dust_Mite_Allergen/3751845","host_type":"repository"},{"url":"http://journals.plos.org/plosone/article/asset?id=10.1371/journal.pone.0160641.PDF","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4993390","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4993390?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Studies on Chitinases and Chitosanases","Allergic Rhinitis and Sensitization","IL-33, ST2, and ILC Pathways","Amino Acid Sequence","Animals","Antibodies","Antigens, Dermatophagoides","Arthropod Proteins","Basophils","Chitin","Cloning, Molecular","Escherichia coli","Female","Gene Expression","Humans","Immune Sera","Male","Protein Binding","Protein Conformation, alpha-Helical","Protein Conformation, beta-Strand","Protein Folding","Protein Interaction Domains and Motifs","Pyroglyphidae","Rabbits","Recombinant Proteins","Respiratory Hypersensitivity","Sequence Alignment","Sequence Homology, Amino Acid"],"mesh_terms":["Protein Conformation, alpha-Helical","Protein Conformation, beta-Strand","Amino Acid Sequence","Animals","Antibodies","Basophils","Chitin","Cloning, Molecular","Escherichia coli","Female","Humans","Immune Sera","Male","Protein Binding","Rabbits","Recombinant Proteins","Respiratory Hypersensitivity","Gene Expression","Sequence Alignment","Sequence Homology, Amino Acid","Protein Folding","Antigens, Dermatophagoides","Pyroglyphidae","Protein Interaction Domains and Motifs","Arthropod Proteins"],"keywords":["Allergen","Basophil activation","House dust mite","Pyroglyphidae","Immunoglobulin E","Chitinase","Basophil","Immunology","Chemistry","Mite","Biology","Allergy","Microbiology","Antibody","Biochemistry","Enzyme","Botany"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"},{"name":"uniprot"},{"name":"nct"},{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T17:11:49.524267Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}