{"doi":"10.1371/journal.pone.0154815","title":"Overrepresentation of IL-10-Expressing B Cells Suppresses Cytotoxic CD4+ T Cell Activity in HBV-Induced Hepatocellular Carcinoma","abstract":null,"journal":"PLOS ONE","year":2016,"id":606980,"datarank":0.6456097639806255,"base_score":4.304065093204169,"endowment":4.304065093204169,"self_citation_contribution":0.6456097639806255,"citation_network_contribution":0.0,"self_endowment_contribution":0.6456097639806255,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":73,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1558410,"name":"Fuhuang Lin","orcid":null,"position":1,"is_corresponding":false},{"id":1558411,"name":"Hongwu Tan","orcid":null,"position":2,"is_corresponding":false},{"id":1558412,"name":"Zun-Qiang Zhu","orcid":null,"position":3,"is_corresponding":false},{"id":1558413,"name":"Zhang-Yun Zhang","orcid":null,"position":4,"is_corresponding":false},{"id":1558414,"name":"Ludong Zhao","orcid":null,"position":5,"is_corresponding":false},{"id":1558409,"name":"Hongwei Xue","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Overrepresentation of IL-10-Expressing B Cells Suppresses Cytotoxic CD4+ T Cell Activity in HBV-Induced Hepatocellular Carcinoma","abstract":"Hepatocellular carcinoma (HCC) is a common cancer with poor prognosis and low five-year survival rate. A strong and effective CD4+ T cell-mediated cytotoxicity was associated with better survival and low recurrence rate in HCC, but the regulatory mechanism that controls CD4+ T cell cytotoxicity in HCC patients is not fully examined. Given that IL-10-expressing B cells could suppress the inflammation of cytotoxic CD8+ T cells, T helper 1 (Th1) cells and Th17 cells, while promoting regulatory T (Treg) cell differentiation, we examined the role of IL-10-expressing B cells in HBV-related HCC patients. We found that compared to healthy controls, HCC patients exhibited significantly higher frequencies of IL-10-expressing B cells, which were negatively correlated with the frequencies of granzyme A, granzyme B, and perforin expressing CD4+ T cells. Surface molecule Tim-1 was preferentially expressed on IL-10-expressing B cells. Therefore, we separated total B cells into Tim-1+ and Tim-1- B cells. CD4+ T cells incubated with Tim-1+ B cells exhibited significantly reduced levels of granzyme A, granzyme B and perforin expression, compared to the CD4+ T cells incubated with Tim-1- B cells. Antagonizing IL-10 in culture rescued CD4+ T cell cytotoxicity. Compared to that in peripheral blood, the level of IL-10-expressing B cells were further upregulated in resected tumor, while the level of CD4+ cytotoxic T cells was downregulated. The negative correlations between IL-10-expressing B cells and CD4+ cytotoxic T cells were also observed in tumor-infiltrating cells. Together, our data revealed an additional antitumor mechanism mediated by IL-10-expressing B cells.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27136203","pmcid":"PMC4852942","openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0154815&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0154815","host_type":"publisher"},{"url":"https://doaj.org/article/a83342a3659a4ad19aaf3c64d5ff8970","host_type":"repository"},{"url":"https://figshare.com/articles/dataset/Overrepresentation_of_IL-10-Expressing_B_Cells_Suppresses_Cytotoxic_CD4_sup_sup_T_Cell_Activity_in_HBV-Induced_Hepatocellular_Carcinoma/3211522","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4852942","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4852942","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4852942?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":[],"mesh_terms":["B-Lymphocytes","CD4-Positive T-Lymphocytes","Th1 Cells","CD8-Positive T-Lymphocytes","Humans","Hepatitis B virus","Carcinoma, Hepatocellular","Liver Neoplasms","Interleukin-10","Flow Cytometry","Adult","Middle Aged","Female","Male","T-Lymphocytes, Regulatory","Granzymes","Perforin","Hepatitis A Virus Cellular Receptor 1"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T05:31:59.780772Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}