{"doi":"10.1371/journal.pone.0144038","title":"Easy and Rapid Detection of Mumps Virus by Live Fluorescent Visualization of Virus-Infected Cells","abstract":null,"journal":"PLOS ONE","year":2015,"id":681844,"datarank":0.4636563680037475,"base_score":3.091042453358316,"endowment":3.091042453358316,"self_citation_contribution":0.4636563680037475,"citation_network_contribution":0.0,"self_endowment_contribution":0.4636563680037475,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1781414,"name":"Takashi Agarikuchi","orcid":null,"position":1,"is_corresponding":false},{"id":1440675,"name":"Yuuki Kurebayashi","orcid":"0000-0002-7523-6160","position":2,"is_corresponding":false},{"id":1781415,"name":"Nona Shibahara","orcid":null,"position":3,"is_corresponding":false},{"id":1781418,"name":"Chihiro Suzuki","orcid":null,"position":4,"is_corresponding":false},{"id":1781420,"name":"Akiko Kishikawa","orcid":null,"position":5,"is_corresponding":false},{"id":1781423,"name":"Keijo Fukushima","orcid":null,"position":6,"is_corresponding":false},{"id":1781426,"name":"Maiko Takano","orcid":null,"position":7,"is_corresponding":false},{"id":1781429,"name":"Fumie Suzuki","orcid":null,"position":8,"is_corresponding":false},{"id":1781430,"name":"Hirohisa Wada","orcid":null,"position":9,"is_corresponding":false},{"id":1781432,"name":"Tadamune Otsubo","orcid":null,"position":10,"is_corresponding":false},{"id":196518,"name":"Kiyoshi Ikeda","orcid":null,"position":11,"is_corresponding":false},{"id":1440678,"name":"Akira Minami","orcid":"0000-0001-8593-941X","position":12,"is_corresponding":false},{"id":819116,"name":"Takashi Suzuki","orcid":"0000-0003-1110-5919","position":13,"is_corresponding":false},{"id":1440679,"name":"Tadanobu Takahashi","orcid":"0000-0001-7601-6066","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Easy and Rapid Detection of Mumps Virus by Live Fluorescent Visualization of Virus-Infected Cells","abstract":"Mumps viruses show diverse cytopathic effects (CPEs) of infected cells and viral plaque formation (no CPE or no plaque formation in some cases) depending on the viral strain, highlighting the difficulty in mumps laboratory studies. In our previous study, a new sialidase substrate, 2-(benzothiazol-2-yl)-4-bromophenyl 5-acetamido-3,5-dideoxy-α-D-glycero-D-galacto-2-nonulopyranosidonic acid (BTP3-Neu5Ac), was developed for visualization of sialidase activity. BTP3-Neu5Ac can easily and rapidly perform histochemical fluorescent visualization of influenza viruses and virus-infected cells without an antiviral antibody and cell fixation. In the present study, the potential utility of BTP3-Neu5Ac for rapid detection of mumps virus was demonstrated. BTP3-Neu5Ac could visualize dot-blotted mumps virus, virus-infected cells, and plaques (plaques should be called focuses due to staining of infected cells in this study), even if a CPE was not observed. Furthermore, virus cultivation was possible by direct pick-up from a fluorescent focus. In conventional methods, visible appearance of the CPE and focuses often requires more than 6 days after infection, but the new method with BTP3-Neu5Ac clearly visualized infected cells after 2 days and focuses after 4 days. The BTP3-Neu5Ac assay is a precise, easy, and rapid assay for confirmation and titration of mumps virus.","is_dataset_classified":null,"base_score":3.091042453358316,"endowment":3.091042453358316,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26629699","pmcid":"PMC4668055","openalex_id":"https://openalex.org/W2261334695","authors":[],"funders":[],"total_grants":0,"fwci":0.8198,"citation_percentile":0.75182625,"influential_citations":0,"citation_trend":[{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":2},{"year":2019,"count":1},{"year":2020,"count":2},{"year":2021,"count":3},{"year":2022,"count":3},{"year":2024,"count":2},{"year":2025,"count":2},{"year":2026,"count":4}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144038&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144038&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0144038","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.pone.0144038","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26629699","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4668055","host_type":"repository"},{"url":"https://doaj.org/article/1fdc325c6b90451db8eb5ddae1940b31","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4668055","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4668055?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Virology and Viral Diseases","Influenza Virus Research Studies","Virus-based gene therapy research","Animals","Chlorocebus aethiops","Fluorescence","Humans","Mumps virus","Vero Cells"],"mesh_terms":["Animals","Chlorocebus aethiops","Fluorescence","Humans","Mumps virus","Vero Cells"],"keywords":["Mumps virus","Virology","Virus","Virus quantification","Biology","Staining","Cell culture","Cytopathic effect","Microbiology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T18:48:10.285076Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}