{"doi":"10.1371/journal.pone.0108808","title":"Development of CpG-Oligodeoxynucleotides for Effective Activation of Rabbit TLR9 Mediated Immune Responses","abstract":null,"journal":"PLoS ONE","year":2014,"id":628452,"datarank":0.4566783656585135,"base_score":3.044522437723423,"endowment":3.044522437723423,"self_citation_contribution":0.4566783656585135,"citation_network_contribution":0.0,"self_endowment_contribution":0.4566783656585135,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1627119,"name":"Chao-Yang Lai","orcid":null,"position":1,"is_corresponding":false},{"id":1627120,"name":"Ping-Hui Tseng","orcid":null,"position":2,"is_corresponding":false},{"id":1627121,"name":"Chiun-Jye Yuan","orcid":null,"position":3,"is_corresponding":false},{"id":1627122,"name":"Li-Chung Hsu","orcid":null,"position":4,"is_corresponding":false},{"id":1627118,"name":"Tsung-Hsien Chuang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Development of CpG-Oligodeoxynucleotides for Effective Activation of Rabbit TLR9 Mediated Immune Responses","abstract":"CpG-oligodeoxynucleotides (CpG-ODN) are potent immune stimuli being developed for use as adjuvants in different species. Toll-like receptor 9 (TLR9) is the cellular receptor for CpG-ODN in mammalian cells. The CpG-ODN with 18-24 deoxynucleotides that are in current use for human and mouse cells, however, have low activity with rabbit TLR9. Using a cell-based activation assay, we developed a type of CpG-ODN containing a GACGTT or AACGTT motif in 12 phosphorothioate-modified deoxynucleotides with potent stimulatory activity for rabbit TLR9. The developed CpG-ODN have higher activities than other developed CpG-ODN in eliciting antigen-nonspecific immune responses in rabbit splenocytes. When mixed with an NJ85 peptide derived from rabbit hemorrhagic disease virus, they had potent activities to boost an antigen-specific T cell activation and antibody production in rabbits. Compared to Freund's adjuvant, the developed CpG-ODN are capable of boosting a potent and less toxic antibody response. The results of this study suggest that both the choice of CpG-motif and its length are important factors for CpG-ODN to effectively activate rabbit TLR9 mediated immune responses.","is_dataset_classified":null,"base_score":3.044522437723423,"endowment":3.044522437723423,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25269083","pmcid":"PMC4182578","openalex_id":"https://openalex.org/W2066124691","authors":[],"funders":[],"total_grants":0,"fwci":0.7284,"citation_percentile":0.68818645,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":2},{"year":2018,"count":1},{"year":2019,"count":2},{"year":2020,"count":4},{"year":2022,"count":1},{"year":2023,"count":3},{"year":2025,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0108808&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0108808&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0108808","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.pone.0108808","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25269083","host_type":"repository"},{"url":"https://doaj.org/article/a8db2ef69c404872b375225b341ba2d5","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4182578","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4182578","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4182578?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Immune Response and Inflammation","Influenza Virus Research Studies","Immune Cell Function and Interaction","Adjuvants, Immunologic","Animals","Antibody Formation","Base Sequence","Cytokines","HEK293 Cells","Hemorrhagic Disease Virus, Rabbit","Humans","Immunoglobulin M","Lymphocyte Activation","Oligodeoxyribonucleotides","Peptides","Phosphates","Rabbits","Spleen","T-Lymphocytes","Toll-Like Receptor 9"],"mesh_terms":["Adjuvants, Immunologic","Animals","Antibody Formation","Base Sequence","Humans","Immunoglobulin M","Lymphocyte Activation","Oligodeoxyribonucleotides","Peptides","Phosphates","Rabbits","Spleen","T-Lymphocytes","Cytokines","Hemorrhagic Disease Virus, Rabbit","Toll-Like Receptor 9","HEK293 Cells"],"keywords":["TLR9","CpG Oligodeoxynucleotide","Toll-Like Receptor 9","Immune system","Adjuvant","Antigen","CpG site","Biology","Molecular biology","Antibody","Immunology","Chemistry","Biochemistry","Gene expression","Gene","DNA methylation"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T13:36:36.133432Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}