{"doi":"10.1371/journal.pone.0063206","title":"Mitochondrial Dynamics Associated with Oxygen-Glucose Deprivation in Rat Primary Neuronal Cultures","abstract":null,"journal":"PLoS ONE","year":2013,"id":661393,"datarank":0.635115975689589,"base_score":4.23410650459726,"endowment":4.23410650459726,"self_citation_contribution":0.635115975689589,"citation_network_contribution":0.0,"self_endowment_contribution":0.635115975689589,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":68,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1726605,"name":"Adam Institoris","orcid":null,"position":1,"is_corresponding":false},{"id":1726606,"name":"Somhrita Dutta","orcid":null,"position":2,"is_corresponding":false},{"id":273764,"name":"Prasad V. G. Katakam","orcid":"0000-0002-4708-9140","position":3,"is_corresponding":false},{"id":209666,"name":"David W. Busija","orcid":null,"position":4,"is_corresponding":false},{"id":1726604,"name":"Edina A. Wappler","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Mitochondrial Dynamics Associated with Oxygen-Glucose Deprivation in Rat Primary Neuronal Cultures","abstract":"Our objective was to investigate the mitochondrial dynamics following oxygen-glucose deprivation (OGD) in cultured rat cortical neurons. We documented changes in morphology, protein expression, and DNA levels in mitochondria following OGD and examined the roles of mitochondrial fission [dynamin-related protein 1 (Drp1), fission protein-1 (Fis1)] and fusion [mitofusin-1 (Mfn1), mitofusin-2 (Mfn2), and optic atrophy-1 protein (OPA1)] proteins on mitochondrial biogenesis and morphogenesis. We tested the effects of two Drp1 blockers [15-deoxy-Δ12,14-Prostaglandin J2 (PGJ2) and Mitochondrial Division Inhibitor (Mdivi-1)] on mitochondrial dynamics and cell survival. One hour of OGD had minimal effects on neuronal viability but mitochondria appeared condensed. Three hours of OGD caused a 60% decrease in neuronal viability accompanied by a transition from primarily normal/tubular and lesser number of rounded mitochondria during normoxia to either poorly labeled or small and large rounded mitochondria. The percentage of rounded mitochondria remained the same. The mitochondrial voltage-dependent anion channel, Complex V, and mitoDNA levels increased after OGD associated with a dramatic reduction in Drp1 expression, less reduction in Mfn2 expression, an increase in Mfn1 expression, with no changes in either OPA1 or Fis1. Although PGJ2 increased polymerization of Drp1, it did not reduce cell death or alter mitochondrial morphology following OGD and Mdivi-1 did not protect neurons against OGD. In summary, mitochondrial biogenesis and maintained fusion occurred in neurons along with mitochondrial fission following OGD; thus Mfn1 but not Drp1 may be a major regulator of these processes.","is_dataset_classified":null,"base_score":4.23410650459726,"endowment":4.23410650459726,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23658809","pmcid":null,"openalex_id":"https://openalex.org/W2009271779","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"3R01HL065380-06S1","title":"Endothelial Dysfunction in Insulin Resistance"},{"funder_name":"National Institutes of Health","grant_id":"2R01HL030260-13A1","title":"REGULATION OF CEREBRAL BLOOD FLOW"},{"funder_name":"National Institutes of Health","grant_id":"5R01HL093554-04","title":"Mitochondrial Influences on Cerebral Arteries"}],"total_grants":3,"fwci":3.7133,"citation_percentile":0.93979395,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":8},{"year":2015,"count":9},{"year":2016,"count":9},{"year":2017,"count":9},{"year":2018,"count":4},{"year":2019,"count":3},{"year":2020,"count":6},{"year":2021,"count":6},{"year":2022,"count":6},{"year":2023,"count":4},{"year":2024,"count":1},{"year":2025,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0063206&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0063206&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0063206","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.pone.0063206","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23658809","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3642144","host_type":"repository"},{"url":"https://doaj.org/article/f90e92b304e24f7087fefeabd750d57f","host_type":"repository"},{"url":"http://dx.doi.org/10.1371/journal.pone.0063206","host_type":""},{"url":"https://dx.doi.org/10.1371/journal.pone.0063206","host_type":""}],"fields_of_study":["Mitochondrial Function and Pathology","Metabolism and Genetic Disorders","ATP Synthase and ATPases Research","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Animals","Cells, Cultured","Female","Glucose","Neurons","Oxygen","Pregnancy","Time Factors","Prostaglandin D2","Rats, Sprague-Dawley","Mitochondrial Proteins","Voltage-Dependent Anion Channels","Rats","Quinazolinones","Mitochondrial Dynamics"],"keywords":["MFN1","MFN2","FIS1","DNM1L","Mitochondrial fission","mitochondrial fusion","Cell biology","Mitochondrion","Mitochondrial biogenesis","Biology","Mitochondrial apoptosis-induced channel","DNAJA3","Mitochondrial DNA","Biochemistry","Inner mitochondrial membrane","Neurons","Time Factors","Prostaglandin D2","Science","Q","R","Mitochondrial Dynamics","Rats","Mitochondrial Proteins","Oxygen","Rats, Sprague-Dawley","Glucose","Pregnancy","Medicine","Animals","Voltage-Dependent Anion Channels","Female","Cells, Cultured","Research Article","Quinazolinones"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T10:55:07.584097Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}