{"doi":"10.1371/journal.pone.0053179","title":"Comprehensive Histone Phosphorylation Analysis and Identification of Pf14-3-3 Protein as a Histone H3 Phosphorylation Reader in Malaria Parasites","abstract":null,"journal":"PLoS ONE","year":2013,"id":649951,"datarank":0.5709993734655481,"base_score":3.8066624897703196,"endowment":3.8066624897703196,"self_citation_contribution":0.5709993734655481,"citation_network_contribution":0.0,"self_endowment_contribution":0.5709993734655481,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":44,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1694594,"name":"Kristina Dzeyk","orcid":null,"position":1,"is_corresponding":false},{"id":471432,"name":"Jeroen Krijgsveld","orcid":"0000-0001-7549-9326","position":2,"is_corresponding":false},{"id":598438,"name":"Nicholas A. Malmquist","orcid":"0000-0003-2822-8245","position":3,"is_corresponding":false},{"id":288360,"name":"Christian Doerig","orcid":"0000-0002-3188-094X","position":4,"is_corresponding":false},{"id":598436,"name":"Artur Scherf","orcid":"0000-0003-2411-3328","position":5,"is_corresponding":false},{"id":1694595,"name":"Jose-Juan Lopez-Rubio","orcid":null,"position":6,"is_corresponding":false},{"id":1694592,"name":"Eeshita G. Dastidar","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Comprehensive Histone Phosphorylation Analysis and Identification of Pf14-3-3 Protein as a Histone H3 Phosphorylation Reader in Malaria Parasites","abstract":"The important role of histone posttranslational modifications, particularly methylation and acetylation, in Plasmodium falciparum gene regulation has been established. However, the role of histone phosphorylation remains understudied. Here, we investigate histone phosphorylation utilizing liquid chromatography and tandem mass spectrometry to analyze histones extracted from asexual blood stages using two improved protocols to enhance preservation of PTMs. Enrichment for phosphopeptides lead to the detection of 14 histone phospho-modifications in P. falciparum. The majority of phosphorylation sites were observed at the N-terminal regions of various histones and were frequently observed adjacent to acetylated lysines. We also report the identification of one novel member of the P. falciparum histone phosphosite binding protein repertoire, Pf14-3-3I. Recombinant Pf14-3-3I protein bound to purified parasite histones. In silico structural analysis of Pf14-3-3 proteins revealed that residues responsible for binding to histone H3 S10ph and/or S28ph are conserved at the primary and the tertiary structure levels. Using a battery of H3 specific phosphopeptides, we demonstrate that Pf14-3-3I preferentially binds to H3S28ph over H3S10ph, independent of modification of neighbouring residues like H3S10phK14ac and H3S28phS32ph. Our data provide key insight into histone phosphorylation sites. The identification of a second member of the histone modification reading machinery suggests a widespread use of histone phosphorylation in the control of various nuclear processes in malaria parasites.","is_dataset_classified":null,"base_score":3.8066624897703196,"endowment":3.8066624897703196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23308157","pmcid":null,"openalex_id":"https://openalex.org/W1983211866","authors":[],"funders":[{"funder_name":"European Commission","grant_id":"215281","title":"Intervention strategies against malaria"},{"funder_name":"European Commission","grant_id":"250320","title":"Monoallelic Gene Expression in Malaria Parasites: A Key Mechanisms in the Pathogen's Survival Strategy"}],"total_grants":2,"fwci":1.9262,"citation_percentile":0.85399181,"influential_citations":0,"citation_trend":[{"year":2013,"count":3},{"year":2014,"count":1},{"year":2015,"count":3},{"year":2016,"count":7},{"year":2017,"count":4},{"year":2018,"count":1},{"year":2019,"count":4},{"year":2020,"count":5},{"year":2021,"count":8},{"year":2022,"count":2},{"year":2024,"count":4},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0053179&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0053179&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0053179","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.pone.0053179","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23308157","host_type":"repository"},{"url":"https://research.monash.edu/en/publications/1825fbb3-43bc-47c0-b5be-93d17c5130b5","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.858.2456","host_type":""},{"url":"https://doaj.org/article/8c250846473d49ea83201137bc952c81","host_type":"repository"},{"url":"https://figshare.com/articles/dataset/Comprehensive_Histone_Phosphorylation_Analysis_and_Identification_of_Pf14_3_3_Protein_as_a_Histone_H3_Phosphorylation_Reader_in_Malaria_Parasites/114884","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3538786","host_type":"repository"},{"url":"http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0053179&representation=PDF","host_type":"repository"},{"url":"http://dx.doi.org/10.1371/journal.pone.0053179","host_type":""},{"url":"https://dx.doi.org/10.1371/journal.pone.0053179","host_type":""}],"fields_of_study":["Ubiquitin and proteasome pathways","Glycosylation and Glycoproteins Research","Signaling Pathways in Disease","0301 basic medicine","03 medical and health sciences","14-3-3 Proteins","Amino Acid Sequence","Erythrocytes","Histones","Humans","Malaria, Falciparum","Models, Molecular","Molecular Sequence Data","Phosphorylation","Plasmodium falciparum","Protein Binding","Protozoan Proteins","Sequence Alignment","Tandem Mass Spectrometry"],"mesh_terms":["Amino Acid Sequence","Erythrocytes","Histones","Humans","Models, Molecular","Molecular Sequence Data","Phosphorylation","Plasmodium falciparum","Protein Binding","Protozoan Proteins","Sequence Alignment","Malaria, Falciparum","14-3-3 Proteins","Tandem Mass Spectrometry"],"keywords":["Histone","Histone H2A","Histone H3","Biology","Phosphorylation","Histone code","Histone methyltransferase","Acetylation","Histone methylation","Histone H1","Biochemistry","SAP30","Cell biology","Molecular biology","Nucleosome","DNA methylation","Gene","Gene expression","Models, Molecular","Erythrocytes","Science","Q","Molecular Sequence Data","Plasmodium falciparum","R","Protozoan Proteins","Histones","14-3-3 Proteins","Tandem Mass Spectrometry","Medicine","Humans","Amino Acid Sequence","Malaria, Falciparum","Sequence Alignment","Research Article","Protein Binding"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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