{"doi":"10.1371/journal.pone.0050296","title":"Immunogenicity of Bivalent Human Papillomavirus DNA Vaccine Using Human Endogenous Retrovirus Envelope-Coated Baculoviral Vectors in Mice and Pigs","abstract":null,"journal":"PLoS ONE","year":2012,"id":648665,"datarank":0.5957730502751685,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"self_citation_contribution":0.4335557636844247,"citation_network_contribution":0.16221728659074375,"self_endowment_contribution":0.4335557636844247,"citer_contribution":0.16221728659074375,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":7,"citers_with_citation_signal":7,"citers_with_endowment":7,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1690636,"name":"Yoon-Ki Hur","orcid":null,"position":1,"is_corresponding":false},{"id":1690637,"name":"Youn-Dong Cho","orcid":null,"position":2,"is_corresponding":false},{"id":1690638,"name":"Mi-Gyeong Kim","orcid":null,"position":3,"is_corresponding":false},{"id":1690639,"name":"Hoon-Taek Lee","orcid":null,"position":4,"is_corresponding":false},{"id":1644360,"name":"Yu-Kyoung Oh","orcid":null,"position":5,"is_corresponding":false},{"id":137079,"name":"Young Bong Kim","orcid":null,"position":6,"is_corresponding":false},{"id":1525268,"name":"Hee-Jung Lee","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Immunogenicity of Bivalent Human Papillomavirus DNA Vaccine Using Human Endogenous Retrovirus Envelope-Coated Baculoviral Vectors in Mice and Pigs","abstract":"Human papillomavirus is known to be the major pathogen of cervical cancer. Here, we report the efficacy of a bivalent human papillomavirus type 16 and 18 DNA vaccine system following repeated dosing in mice and pigs using a recombinant baculovirus bearing human endogenous retrovirus envelope protein (AcHERV) as a vector. The intramuscular administration of AcHERV-based HPV16L1 and HPV18L1 DNA vaccines induced antigen-specific serum IgG, vaginal IgA, and neutralizing antibodies to levels comparable to those achieved using the commercially marketed vaccine Cervarix. Similar to Cervarix, AcHERV-based bivalent vaccinations completely blocked subsequent vaginal challenge with HPV type-specific pseudovirions. However, AcHERV-based bivalent vaccinations induced significantly higher cell-mediated immune responses than Cervarix, promoting 4.5- (HPV16L1) and 3.9-(HPV18L1) fold higher interferon-γ production in splenocytes upon stimulation with antigen type-specific pseudovirions. Repeated dosing did not affect the immunogenicity of AcHERV DNA vaccines. Three sequential immunizations with AcHERV-HP18L1 DNA vaccine followed by three repeated dosing with AcHERV-HP16L1 over 11 weeks induced an initial production of anti-HPV18L1 antibody followed by subsequent induction of anti-HPV16L1 antibody. Finally, AcHERV-based bivalent DNA vaccination induced antigen-specific serum IgG immune responses in pigs. These results support the further development of AcHERV as a bivalent human papillomavirus DNA vaccine system for use in preventing the viral infection as well as treating the infected women by inducing both humoral and cell-mediated immune responses. Moreover, the possibility of repeated dosing indicates the utility of AcHERV system for reusable vectors of other viral pathogen vaccines.","is_dataset_classified":null,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23209698","pmcid":"PMC3507738","openalex_id":"https://openalex.org/W1993044863","authors":[],"funders":[],"total_grants":0,"fwci":1.499,"citation_percentile":0.82551933,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":2},{"year":2015,"count":5},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2019,"count":1},{"year":2020,"count":3},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0050296&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0050296&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0050296","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.pone.0050296","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23209698","host_type":"repository"},{"url":"https://doaj.org/article/f6853d5e11da4ca0b9e35eb192161b84","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3507738","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3507738","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3507738?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Virus-based gene therapy research","Viral Infectious Diseases and Gene Expression in Insects","Immunotherapy and Immune Responses","Animals","Baculoviridae","Endogenous Retroviruses","Enzyme-Linked Immunosorbent Assay","Female","Genetic Vectors","Green Fluorescent Proteins","Human papillomavirus 16","Human papillomavirus 18","Humans","Mice","Mice, Inbred BALB C","Neutralization Tests","Papillomavirus Vaccines","Retroviridae","Swine","Vaccines, DNA"],"mesh_terms":["Animals","Enzyme-Linked Immunosorbent Assay","Female","Genetic Vectors","Humans","Mice, Inbred BALB C","Neutralization Tests","Retroviridae","Swine","Baculoviridae","Vaccines, DNA","Endogenous Retroviruses","Green Fluorescent Proteins","Mice","Human papillomavirus 18","Human papillomavirus 16","Papillomavirus Vaccines"],"keywords":["DNA vaccination","Immunogenicity","Bivalent (engine)","Virology","Vaccination","Immune system","Antigen","Biology","Immunology","Gardasil","Antibody","Medicine","Immunization","Cervical cancer","Chemistry","Cancer"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T02:51:10.426312Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}