{"doi":"10.1371/journal.pone.0040826","title":"Respiratory Syncytial Virus Assembles into Structured Filamentous Virion Particles Independently of Host Cytoskeleton and Related Proteins","abstract":null,"journal":"PLoS ONE","year":2012,"id":606342,"datarank":0.5606504427425053,"base_score":3.7376696182833684,"endowment":3.7376696182833684,"self_citation_contribution":0.5606504427425053,"citation_network_contribution":0.0,"self_endowment_contribution":0.5606504427425053,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":41,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1556600,"name":"Thomas J. Utley","orcid":null,"position":1,"is_corresponding":false},{"id":1556602,"name":"Ryan E. Craven","orcid":null,"position":2,"is_corresponding":false},{"id":518095,"name":"Meredith C. Rogers","orcid":"0000-0003-1798-6294","position":3,"is_corresponding":false},{"id":879554,"name":"Lynne A. Lapierre","orcid":"0000-0002-7163-0830","position":4,"is_corresponding":false},{"id":86851,"name":"James R. Goldenring","orcid":"0000-0002-7833-2940","position":5,"is_corresponding":false},{"id":106531,"name":"James E. Crowe","orcid":"0000-0002-0049-1079","position":6,"is_corresponding":false},{"id":666236,"name":"Fyza Y. Shaikh","orcid":"0000-0001-7839-8483","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Respiratory Syncytial Virus Assembles into Structured Filamentous Virion Particles Independently of Host Cytoskeleton and Related Proteins","abstract":"Respiratory syncytial virus (RSV) is a single-stranded RNA virus that assembles into viral filaments at the cell surface. Virus assembly often depends on the ability of a virus to use host proteins to accomplish viral tasks. Since the fusion protein cytoplasmic tail (FCT) is critical for viral filamentous assembly, we hypothesized that host proteins important for viral assembly may be recruited by the FCT. Using a yeast two-hybrid screen, we found that filamin A interacted with FCT, and mammalian cell experiments showed it localized to viral filaments but did not affect viral replication. Furthermore, we found that a number of actin-associated proteins also were excluded from viral filaments. Actin or tubulin cytoskeletal rearrangement was not necessary for F trafficking to the cell surface or for viral assembly into filaments, but was necessary for optimal viral replication and may be important for anchoring viral filaments. These findings suggest that RSV assembly into filaments occurs independently of actin polymerization and that viral proteins are the principal drivers for the mechanical tasks involved with formation of complex, structured RSV filaments at the host cell plasma membrane.","is_dataset_classified":null,"base_score":3.7376696182833684,"endowment":3.7376696182833684,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22808269","pmcid":"PMC3396619","openalex_id":"https://openalex.org/W2052492915","authors":[],"funders":[{"funder_name":"NIDDK NIH HHS","grant_id":"DK58404","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"DK20593","title":null},{"funder_name":"PHS HHS","grant_id":"NIGMS/NIH T32 GM007347","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P30 CA68485","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P30CA068485","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA68485","title":null},{"funder_name":"NEI NIH HHS","grant_id":"P30 EY008126","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"DK59637","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"P30 DK020593","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"P30 DK058404","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"R01 DK070856","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"DK058404","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"P60 DK020593","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"R01 DK048370","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM007347","title":null},{"funder_name":"NEI NIH HHS","grant_id":"EY08126","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"U24 DK059637","title":null},{"funder_name":"NICHD NIH HHS","grant_id":"HD15052","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"NIH R01 DK48370","title":null},{"funder_name":"NICHD NIH HHS","grant_id":"P30 HD015052","title":null},{"funder_name":"National Institutes of Health","grant_id":"3P30CA068485-21S4","title":"Cancer Center Support Grant"},{"funder_name":"National Institutes of Health","grant_id":"1U24DK059637-01","title":"CORE--METABOLIC PATHOPHYSIOLOGY"},{"funder_name":"National Institutes of Health","grant_id":"5R01DK070856-03","title":"Molecular characteristics of the apical recycling system"},{"funder_name":"National Institutes of Health","grant_id":"5P60DK020593-25","title":"Diabetes Research and Training Center"},{"funder_name":"National Institutes of Health","grant_id":"3P30CA068485-23S2","title":"Cancer Center Support Grant"},{"funder_name":"National Institutes of Health","grant_id":"3R01DK048370-09S1","title":"SMALL GTP BINDING PROTEINS IN GASTROINTESTINAL MUCOSA"},{"funder_name":"National Institutes of Health","grant_id":"1P30DK058404-01A1","title":"CORE--MICROARRAY"},{"funder_name":"National Institutes of Health","grant_id":"5P30HD015052-08","title":"MEDIA SERVICES CORE"},{"funder_name":"National Institutes of Health","grant_id":"5T32GM007347-08","title":"MEDICAL SCIENTIST TRAINING PROGRAM"}],"total_grants":29,"fwci":2.3292,"citation_percentile":0.87836625,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":6},{"year":2014,"count":2},{"year":2015,"count":3},{"year":2016,"count":5},{"year":2017,"count":4},{"year":2018,"count":2},{"year":2019,"count":1},{"year":2020,"count":4},{"year":2021,"count":2},{"year":2022,"count":6},{"year":2024,"count":2},{"year":2025,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0040826&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0040826&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0040826","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.pone.0040826","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22808269","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.782.7174","host_type":""},{"url":"https://doaj.org/article/974d60faea5648759a990536d9eda991","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3396619","host_type":"repository"},{"url":"https://figshare.com/articles/dataset/Respiratory_Syncytial_Virus_Assembles_into_Structured_Filamentous_Virion_Particles_Independently_of_Host_Cytoskeleton_and_Related_Proteins/122732","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3396619","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3396619?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1371/journal.pone.0040826","host_type":""},{"url":"https://dx.doi.org/10.1371/journal.pone.0040826","host_type":""}],"fields_of_study":["Respiratory viral infections research","Plant Virus Research Studies","Bacteriophages and microbial interactions","0301 basic medicine","03 medical and health sciences","Animals","Cell Line","Contractile Proteins","Cytoskeletal Proteins","Cytoskeleton","Filamins","Gene Expression Regulation","Gene Knockdown Techniques","Host-Pathogen Interactions","Humans","Microfilament Proteins","Protein Transport","Respiratory Syncytial Viruses","Two-Hybrid System Techniques","Virion","Virus Assembly"],"mesh_terms":["Animals","Cell Line","Contractile Proteins","Cytoskeletal Proteins","Cytoskeleton","Gene Expression Regulation","Humans","Microfilament Proteins","Respiratory Syncytial Viruses","Virion","Virus Assembly","Two-Hybrid System Techniques","Protein Transport","Host-Pathogen Interactions","Gene Knockdown Techniques","Filamins"],"keywords":["Viral entry","Cell biology","Biology","Viral structural protein","Cytoskeleton","Viral replication","Virus","Actin","Viral protein","Cytoplasm","Virology","Cell","Genetics","Science","Filamins","Virus Assembly","Q","Microfilament Proteins","R","Virion","Cell Line","Respiratory Syncytial Viruses","Cytoskeletal Proteins","Protein Transport","Contractile Proteins","Gene Expression Regulation","Gene Knockdown Techniques","Two-Hybrid System Techniques","Host-Pathogen Interactions","Medicine","Animals","Humans","Research Article"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T04:21:00.967196Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}