{"doi":"10.1371/journal.pone.0039329","title":"Glycogen Synthase Kinase 3 (GSK3) Inhibitor, SB-216763, Promotes Pluripotency in Mouse Embryonic Stem Cells","abstract":null,"journal":"PLoS ONE","year":2012,"id":650350,"datarank":0.5897738449086489,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"self_citation_contribution":0.5897738449086489,"citation_network_contribution":0.0,"self_endowment_contribution":0.5897738449086489,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":50,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1695717,"name":"Jason T. Schott","orcid":null,"position":1,"is_corresponding":false},{"id":734254,"name":"Brenda L. Noble","orcid":null,"position":2,"is_corresponding":false},{"id":410088,"name":"Daniel C. Mendez","orcid":"0000-0002-0901-2927","position":3,"is_corresponding":false},{"id":1695720,"name":"Paul S. Caseley","orcid":null,"position":4,"is_corresponding":false},{"id":1695722,"name":"Sarah C. Peterson","orcid":null,"position":5,"is_corresponding":false},{"id":1695724,"name":"Tyler J. Routledge","orcid":null,"position":6,"is_corresponding":false},{"id":1695726,"name":"Nilay V. Patel","orcid":null,"position":7,"is_corresponding":false},{"id":849756,"name":"Leslie A. Kirby","orcid":"0000-0003-4467-2661","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Glycogen Synthase Kinase 3 (GSK3) Inhibitor, SB-216763, Promotes Pluripotency in Mouse Embryonic Stem Cells","abstract":"Canonical Wnt/β-catenin signaling has been suggested to promote self-renewal of pluripotent mouse and human embryonic stem cells. Here, we show that SB-216763, a glycogen synthase kinase-3 (GSK3) inhibitor, can maintain mouse embryonic stem cells (mESCs) in a pluripotent state in the absence of exogenous leukemia inhibitory factor (LIF) when cultured on mouse embryonic fibroblasts (MEFs). MESCs maintained with SB-216763 for one month were morphologically indistinguishable from LIF-treated mESCs and expressed pluripotent-specific genes Oct4, Sox2, and Nanog. Furthermore, Nanog immunostaining was more homogenous in SB-216763-treated colonies compared to LIF. Embryoid bodies (EBs) prepared from these mESCs expressed early-stage markers for all three germ layers, and could efficiently differentiate into cardiac-like cells and MAP2-immunoreactive neurons. To our knowledge, SB-216763 is the first GSK3 inhibitor that can promote self-renewal of mESC co-cultured with MEFs for more than two months.","is_dataset_classified":null,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22745733","pmcid":"PMC3383737","openalex_id":"https://openalex.org/W2076549991","authors":[],"funders":[{"funder_name":"National Science Foundation","grant_id":"0737149","title":"Implementation of an Integrative Cell Culture and Stem Cell Laboratory Course at California State University, Fullerton"},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null}],"total_grants":3,"fwci":1.9663,"citation_percentile":0.86187372,"influential_citations":0,"citation_trend":[{"year":2013,"count":2},{"year":2014,"count":8},{"year":2015,"count":6},{"year":2016,"count":6},{"year":2017,"count":3},{"year":2018,"count":2},{"year":2019,"count":3},{"year":2020,"count":5},{"year":2021,"count":3},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":3},{"year":2025,"count":2},{"year":2026,"count":4}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0039329&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0039329&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0039329","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.pone.0039329","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22745733","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.793.1547","host_type":""},{"url":"https://doaj.org/article/d28c02334bc04b5f949eb4367e1f0a2f","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3383737","host_type":"repository"},{"url":"https://figshare.com/articles/Glycogen_Synthase_Kinase_3_GSK3_Inhibitor_SB_216763_Promotes_Pluripotency_in_Mouse_Embryonic_Stem_Cells/123479","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3383737","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3383737?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1371/journal.pone.0039329","host_type":""},{"url":"https://dx.doi.org/10.1371/journal.pone.0039329","host_type":""}],"fields_of_study":["Pluripotent Stem Cells Research","Tissue Engineering and Regenerative Medicine","CRISPR and Genetic Engineering","0301 basic medicine","03 medical and health sciences","Animals","Cell Differentiation","Cell Line","Embryonic Stem Cells","Glycogen Synthase Kinase 3","Humans","Immunohistochemistry","Indoles","Maleimides","Mice","Neurons","Polymerase Chain Reaction","beta Catenin"],"mesh_terms":["Animals","Cell Differentiation","Cell Line","Humans","Immunohistochemistry","Indoles","Maleimides","Neurons","Polymerase Chain Reaction","Glycogen Synthase Kinase 3","beta Catenin","Mice","Embryonic Stem Cells"],"keywords":["Homeobox protein NANOG","Embryoid body","Embryonic stem cell","Rex1","SOX2","Induced pluripotent stem cell","GSK-3","Leukemia inhibitory factor","Biology","Stem cell","KOSR","Cell biology","Molecular biology","Wnt signaling pathway","Chemistry","Kinase","Signal transduction","Biochemistry","Gene","Neurons","Indoles","Science","Q","R","Cell Differentiation","Immunohistochemistry","Polymerase Chain Reaction","Cell Line","Maleimides","Glycogen Synthase Kinase 3","Mice","Medicine","Animals","Humans","Embryonic Stem Cells","beta Catenin","Research Article"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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