{"doi":"10.1371/journal.pone.0027070","title":"Expansion of Intestinal Epithelial Stem Cells during Murine Development","abstract":null,"journal":"PLoS ONE","year":2011,"id":649116,"datarank":0.6646225198264971,"base_score":4.430816798843313,"endowment":4.430816798843313,"self_citation_contribution":0.6646225198264971,"citation_network_contribution":0.0,"self_endowment_contribution":0.6646225198264971,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":83,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":607955,"name":"Aaron P. Garrison","orcid":"0000-0003-2327-711X","position":1,"is_corresponding":false},{"id":1692025,"name":"Karen E. Speck","orcid":null,"position":2,"is_corresponding":false},{"id":383325,"name":"Christopher M. Dekaney","orcid":"0000-0002-3252-1199","position":3,"is_corresponding":false},{"id":593262,"name":"Laurianne Van Landeghem","orcid":"0000-0001-7408-3521","position":4,"is_corresponding":false},{"id":769067,"name":"Xiaofei Sun","orcid":"0000-0002-4835-9309","position":5,"is_corresponding":false},{"id":1692029,"name":"Susan J. Henning","orcid":null,"position":6,"is_corresponding":false},{"id":301418,"name":"Michael A. Helmrath","orcid":"0000-0003-3112-7541","position":7,"is_corresponding":false},{"id":597293,"name":"Jeffrey J. Dehmer","orcid":"0000-0003-3061-4188","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Expansion of Intestinal Epithelial Stem Cells during Murine Development","abstract":"Murine small intestinal crypt development is initiated during the first postnatal week. Soon after formation, overall increases in the number of crypts occurs through a bifurcating process called crypt fission, which is believed to be driven by developmental increases in the number of intestinal stem cells (ISCs). Recent evidence suggests that a heterogeneous population of ISCs exists within the adult intestine. Actively cycling ISCs are labeled by Lgr5, Ascl2 and Olfm4; whereas slowly cycling or quiescent ISC are marked by Bmi1 and mTert. The goal of this study was to correlate the expression of these markers with indirect measures of ISC expansion during development, including quantification of crypt fission and side population (SP) sorting. Significant changes were observed in the percent of crypt fission and SP cells consistent with ISC expansion between postnatal day 14 and 21. Quantitative real-time polymerase chain reaction (RT-PCR) for the various ISC marker mRNAs demonstrated divergent patterns of expression. mTert surged earliest, during the first week of life as crypts are initially being formed, whereas Lgr5 and Bmi1 peaked on day 14. Olfm4 and Ascl2 had variable expression patterns. To assess the number and location of Lgr5-expressing cells during this period, histologic sections from intestines of Lgr5-EGFP mice were subjected to quantitative analysis. There was attenuated Lgr5-EGFP expression at birth and through the first week of life. Once crypts were formed, the overall number and percent of Lgr5-EGFP positive cells per crypt remain stable throughout development and into adulthood. These data were supported by Lgr5 in situ hybridization in wild-type mice. We conclude that heterogeneous populations of ISCs are expanding as measured by SP sorting and mRNA expression at distinct developmental time points.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22102874","pmcid":"PMC3213109","openalex_id":null,"authors":[],"funders":[{"funder_name":"NIDDK NIH HHS","grant_id":"R01 DK083325","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"R01-DK083325","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM008450","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"U01 DK085532","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"U01 DK085547","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"P30 DK034987","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"U24 DK085532","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32-GM008450","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"U01-DK085547","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01DK083325-03","title":"Mechanisms of Intestinal Stem Cell Expansion Following Resection"},{"funder_name":"National Institutes of Health","grant_id":"2T32GM008450-26","title":"Trauma Research Fellowship"},{"funder_name":"National Institutes of Health","grant_id":"3U01DK085547-03S1","title":"Collaborative Approaches to the Study of Intestinal Epithelial Stem Cells"},{"funder_name":"National Institutes of Health","grant_id":"5P30DK034987-28","title":"Administrative Core and Enrichment Program"}],"total_grants":13,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0027070&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0027070","host_type":"publisher"},{"url":"https://doaj.org/article/20867ca9147547bdb19675089fa68876","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3213109","host_type":"repository"},{"url":"https://figshare.com/articles/Expansion_of_Intestinal_Epithelial_Stem_Cells_during_Murine_Development/131513","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3213109","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3213109?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1371/journal.pone.0027070","host_type":""},{"url":"https://dx.doi.org/10.17615/kr0r-8653","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/22102874","host_type":""},{"url":"http://dx.doi.org/10.1371/journal.pone.0027070","host_type":""},{"url":"https://dx.doi.org/10.1371/journal.pone.0027070","host_type":""}],"fields_of_study":["0301 basic medicine","03 medical and health sciences"],"mesh_terms":["Intestines","Intestinal Mucosa","Epithelial Cells","Stem Cells","Animals","Mice, Inbred C57BL","Mice","Green Fluorescent Proteins","Receptors, G-Protein-Coupled","RNA, Messenger","In Situ Hybridization","Cell Lineage","Female","Male","Real-Time Polymerase Chain Reaction","Biomarkers"],"keywords":["Male","Science","Stem Cells","Q","Green Fluorescent Proteins","R","Epithelial Cells","Real-Time Polymerase Chain Reaction","Receptors, G-Protein-Coupled","Intestines","Mice, Inbred C57BL","Mice","Medicine","Animals","Cell Lineage","Female","RNA, Messenger","Intestinal Mucosa","Biomarkers","In Situ Hybridization","Research Article"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"refseq"},{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T03:18:17.987727Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}