{"doi":"10.1371/journal.pone.0012229","title":"DNA Polymerases β and λ Mediate Overlapping and Independent Roles in Base Excision Repair in Mouse Embryonic Fibroblasts","abstract":null,"journal":"PLoS ONE","year":2010,"id":662761,"datarank":0.6698862177981877,"base_score":4.465908118654584,"endowment":4.465908118654584,"self_citation_contribution":0.6698862177981877,"citation_network_contribution":0.0,"self_endowment_contribution":0.6698862177981877,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":86,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1730291,"name":"Padmini S. Kedar","orcid":null,"position":1,"is_corresponding":false},{"id":376176,"name":"Deborah J. Stumpo","orcid":"0009-0008-4387-8776","position":2,"is_corresponding":false},{"id":1189243,"name":"Barbara Bertocci","orcid":"0000-0002-3653-3576","position":3,"is_corresponding":false},{"id":370977,"name":"Jonathan H. Freedman","orcid":"0000-0002-6118-0290","position":4,"is_corresponding":false},{"id":694572,"name":"Leona D. Samson","orcid":null,"position":5,"is_corresponding":false},{"id":170499,"name":"Samuel H. Wilson","orcid":null,"position":6,"is_corresponding":false},{"id":594356,"name":"Elena K. Braithwaite","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"DNA Polymerases β and λ Mediate Overlapping and Independent Roles in Base Excision Repair in Mouse Embryonic Fibroblasts","abstract":"Base excision repair (BER) is a DNA repair pathway designed to correct small base lesions in genomic DNA. While DNA polymerase beta (pol beta) is known to be the main polymerase in the BER pathway, various studies have implicated other DNA polymerases in back-up roles. One such polymerase, DNA polymerase lambda (pol lambda), was shown to be important in BER of oxidative DNA damage. To further explore roles of the X-family DNA polymerases lambda and beta in BER, we prepared a mouse embryonic fibroblast cell line with deletions in the genes for both pol beta and pol lambda. Neutral red viability assays demonstrated that pol lambda and pol beta double null cells were hypersensitive to alkylating and oxidizing DNA damaging agents. In vitro BER assays revealed a modest contribution of pol lambda to single-nucleotide BER of base lesions. Additionally, using co-immunoprecipitation experiments with purified enzymes and whole cell extracts, we found that both pol lambda and pol beta interact with the upstream DNA glycosylases for repair of alkylated and oxidized DNA bases. Such interactions could be important in coordinating roles of these polymerases during BER.","is_dataset_classified":null,"base_score":4.465908118654584,"endowment":4.465908118654584,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20805875","pmcid":"PMC2923601","openalex_id":"https://openalex.org/W2028468016","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"R01 CA075576","title":null},{"funder_name":"PHS HHS","grant_id":"HHSN273200700046U","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA055042","title":null},{"funder_name":"Intramural NIH HHS","grant_id":"Z01 ES050158","title":null},{"funder_name":"Intramural NIH HHS","grant_id":"Z01 ES050159","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA055042","title":null},{"funder_name":"National Institutes of Health","grant_id":"1Z01ES050158-10","title":"Mechanisms of Nucleic Acid Enzymes - Pol Beta and Mammal"},{"funder_name":"National Institutes of Health","grant_id":"1Z01ES050159-08","title":"Biology of Mammalian Base Excision Repair"}],"total_grants":8,"fwci":2.9891,"citation_percentile":0.91554807,"influential_citations":0,"citation_trend":[{"year":2012,"count":10},{"year":2013,"count":8},{"year":2014,"count":9},{"year":2015,"count":4},{"year":2016,"count":2},{"year":2017,"count":8},{"year":2018,"count":3},{"year":2019,"count":6},{"year":2020,"count":5},{"year":2021,"count":7},{"year":2022,"count":2},{"year":2023,"count":5},{"year":2024,"count":5},{"year":2025,"count":3},{"year":2026,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0012229&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0012229&type=printable","host_type":"publisher"},{"url":"http://dx.plos.org/10.1371/journal.pone.0012229","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.pone.0012229","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20805875","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2923601","host_type":"repository"},{"url":"https://doaj.org/article/39f1c48a89724a2ba4c79f86b70e38f8","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2923601","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2923601?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1371/journal.pone.0012229","host_type":""},{"url":"https://dx.doi.org/10.1371/journal.pone.0012229","host_type":""},{"url":"https://hdl.handle.net/1721.1/66510","host_type":""},{"url":"https://doi.org/https://doi.org/10.1371/journal.pone.0012229","host_type":""}],"fields_of_study":["DNA Repair Mechanisms","Carcinogens and Genotoxicity Assessment","DNA and Nucleic Acid Chemistry","0301 basic medicine","0303 health sciences","03 medical and health sciences","Animals","Cell Line","Cell Survival","DNA Damage","DNA Glycosylases","DNA Polymerase beta","DNA Repair","Embryo, Mammalian","Fibroblasts","Gene Knockout Techniques","Humans","Mice"],"mesh_terms":["Animals","Cell Line","Cell Survival","DNA Damage","DNA Repair","Embryo, Mammalian","Fibroblasts","Humans","DNA Polymerase beta","DNA Glycosylases","Mice","Gene Knockout Techniques"],"keywords":["Base excision repair","DNA polymerase","DNA polymerase beta","Molecular biology","Biology","DNA polymerase mu","DNA polymerase II","Polymerase","DNA repair","DNA polymerase delta","DNA damage","DNA","Processivity","DNA clamp","Genetics","Gene","Polymerase chain reaction","Circular bacterial chromosome","Reverse transcriptase","Cell Survival","Science","Q","R","Fibroblasts","Embryo, Mammalian","Cell Line","DNA Glycosylases","Gene Knockout Techniques","Mice","Medicine","Animals","Humans","Research Article"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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