{"doi":"10.1371/journal.pntd.0010442","title":"A recombinant monoclonal-based Taenia antigen assay that reflects disease activity in extra-parenchymal neurocysticercosis","abstract":"BACKGROUND: Antigen tests for diagnosis and disease monitoring in some types of neurocysticercosis (NCC) are useful but access to testing has been limited by availability of proprietary reagents and/or kits. METHODS/PRINCIPAL FINDINGS: Three previously identified IgM-secreting hybridomas whose IgM products demonstrated specificity to Taenia solium underwent variable heavy and light chain sequencing and isotype conversion to mouse IgG. Screening of these recombinantly expressed IgG anti-Ts hybridomas, identified one (TsG10) with the highest affinity to crude Taenia antigen. TsG10 was then used as a capture antibody in a sandwich antigen detection immunoassay in combination with either a high titer polyclonal anti-Ts antibody or with biotinylated TsG10 (termed TsG10*bt). Using serum, plasma, and CSF samples from patients with active NCC and those from NCC-uninfected patients, ROC curve analyses demonstrated that the TsG10-TsG10-*bt assay achieved a 98% sensitivity and 100% specificity in detecting samples known to be antigen positive and outperformed the polyclonal based assay (sensitivity of 93% with 100% specificity). By comparing levels of Ts antigen (Ag) in paired CSF (n = 10) or plasma/serum (n = 19) samples from well-characterized patients with extra-parenchymal NCC early in infection and at the time of definitive cure, all but 2 (1 from CSF and 1 from plasma) became undetectable. There was a high degree of correlation (r = 0.98) between the Ag levels detected by this new assay and levels found by a commercial assay. Pilot studies indicate that this antigen can be detected in the urine of patients with active NCC. CONCLUSIONS/SIGNIFICANCE: A newly developed recombinant monoclonal antibody-based Ts Ag detection immunoassay is extremely sensitive in the detection of extra-parenchymal NCC and can be used to monitor the success of treatment in the CSF, serum/plasma and urine. The ability to produce recombinant TsG10 at scale should enable use of this antigen detection immunoassay wherever NCC is endemic. CLINICAL TRIAL REGISTRATION: ClinicalTrials.gov Identifiers: NCT00001205 - & NCT00001645.","journal":"PLoS neglected tropical diseases","year":2022,"id":258694,"datarank":0.6628657774954874,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"self_citation_contribution":0.4335557636844247,"citation_network_contribution":0.22931001381106267,"self_endowment_contribution":0.4335557636844247,"citer_contribution":0.22931001381106267,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":10,"citers_with_citation_signal":7,"citers_with_endowment":7,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9683,"is_data_producer":true,"deposit_databanks":{"ClinicalTrials.gov":["NCT00001205","NCT00001645"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":718153,"name":"Joshua Sciurba","orcid":"0000-0002-2499-1713","position":1,"is_corresponding":false},{"id":910783,"name":"Jiana Blaha","orcid":null,"position":2,"is_corresponding":false},{"id":910275,"name":"Siddhartha Mahanty","orcid":"0000-0003-1068-0524","position":3,"is_corresponding":false},{"id":910276,"name":"Adriana Contreras‐Paredes","orcid":"0000-0001-9965-4108","position":4,"is_corresponding":false},{"id":455137,"name":"Héctor H. Garcı́a","orcid":"0000-0002-4633-6269","position":5,"is_corresponding":false},{"id":323516,"name":"Theodore E. Nash","orcid":"0000-0003-4619-0068","position":6,"is_corresponding":false},{"id":287794,"name":"Thomas B. Nutman","orcid":"0000-0001-6887-4941","position":7,"is_corresponding":false},{"id":323512,"name":"Elise M. O’Connell","orcid":"0000-0001-9425-2011","position":8,"is_corresponding":false},{"id":910782,"name":"Madelynn Corda","orcid":null,"position":0,"is_corresponding":true}],"reference_count":17,"raw_metadata":null,"created_at":"2026-07-19T00:25:43.037820Z","pmid":"35617367","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}