{"doi":"10.1371/journal.pgen.1009799","title":"The chromatin remodeling protein CHD-1 and the EFL-1/DPL-1 transcription factor cooperatively down regulate CDK-2 to control SAS-6 levels and centriole number","abstract":"Centrioles are submicron-scale, barrel-shaped organelles typically found in pairs, and play important roles in ciliogenesis and bipolar spindle assembly. In general, successful execution of centriole-dependent processes is highly reliant on the ability of the cell to stringently control centriole number. This in turn is mainly achieved through the precise duplication of centrioles during each S phase. Aberrations in centriole duplication disrupt spindle assembly and cilia-based signaling and have been linked to cancer, primary microcephaly and a variety of growth disorders. Studies aimed at understanding how centriole duplication is controlled have mainly focused on the post-translational regulation of two key components of this pathway: the master regulatory kinase ZYG-1/Plk4 and the scaffold component SAS-6. In contrast, how transcriptional control mechanisms might contribute to this process have not been well explored. Here we show that the chromatin remodeling protein CHD-1 contributes to the regulation of centriole duplication in the C. elegans embryo. Specifically, we find that loss of CHD-1 or inactivation of its ATPase activity can restore embryonic viability and centriole duplication to a strain expressing insufficient ZYG-1 activity. Interestingly, loss of CHD-1 is associated with increases in the levels of two ZYG-1-binding partners: SPD-2, the centriole receptor for ZYG-1 and SAS-6. Finally, we explore transcriptional regulatory networks governing centriole duplication and find that CHD-1 and a second transcription factor, EFL-1/DPL-1 cooperate to down regulate expression of CDK-2, which in turn promotes SAS-6 protein levels. Disruption of this regulatory network results in the overexpression of SAS-6 and the production of extra centrioles.","journal":"PLoS Genetics","year":2022,"id":265795,"datarank":0.3596842909197557,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.0,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9505,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":829378,"name":"Lindsey K Gentry","orcid":"0000-0002-7934-4652","position":1,"is_corresponding":false},{"id":829379,"name":"Mary Bergwell","orcid":"0000-0003-2139-6756","position":2,"is_corresponding":false},{"id":829380,"name":"Amy Smith","orcid":"0000-0003-1991-3216","position":3,"is_corresponding":false},{"id":925391,"name":"Sarah E. Guagliardo","orcid":null,"position":4,"is_corresponding":false},{"id":532095,"name":"Peter Kropp","orcid":"0000-0002-2977-6123","position":5,"is_corresponding":false},{"id":829381,"name":"Prabhu Sankaralingam","orcid":"0000-0001-9801-0845","position":6,"is_corresponding":false},{"id":363323,"name":"Yan Liu","orcid":"0000-0001-5683-8695","position":7,"is_corresponding":false},{"id":812805,"name":"Eric Spooner","orcid":null,"position":8,"is_corresponding":false},{"id":378241,"name":"Bruce Bowerman","orcid":"0000-0002-6479-8707","position":9,"is_corresponding":false},{"id":829382,"name":"Kevin O‘Connell","orcid":"0000-0002-9789-288X","position":10,"is_corresponding":false},{"id":829377,"name":"Jyoti Iyer","orcid":"0000-0002-0942-6919","position":0,"is_corresponding":true}],"reference_count":95,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:26:49.862989Z","pmid":"35377871","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}