{"doi":"10.1371/journal.pgen.1008968","title":"Phosphoregulation of HORMA domain protein HIM-3 promotes asymmetric synaptonemal complex disassembly in meiotic prophase in Caenorhabditis elegans","abstract":"In the two cell divisions of meiosis, diploid genomes are reduced into complementary haploid sets through the discrete, two-step removal of chromosome cohesion, a task carried out in most eukaryotes by protecting cohesion at the centromere until the second division. In eukaryotes without defined centromeres, however, alternative strategies have been innovated. The best-understood of these is found in the nematode Caenorhabditis elegans: after the single off-center crossover divides the chromosome into two segments, or arms, several chromosome-associated proteins or post-translational modifications become specifically partitioned to either the shorter or longer arm, where they promote the correct timing of cohesion loss through as-yet unknown mechanisms. Here, we investigate the meiotic axis HORMA-domain protein HIM-3 and show that it becomes phosphorylated at its C-terminus, within the conserved \"closure motif\" region bound by the related HORMA-domain proteins HTP-1 and HTP-2. Binding of HTP-2 is abrogated by phosphorylation of the closure motif in in vitro assays, strongly suggesting that in vivo phosphorylation of HIM-3 likely modulates the hierarchical structure of the chromosome axis. Phosphorylation of HIM-3 only occurs on synapsed chromosomes, and similarly to other previously-described phosphorylated proteins of the synaptonemal complex, becomes restricted to the short arm after designation of crossover sites. Regulation of HIM-3 phosphorylation status is required for timely disassembly of synaptonemal complex central elements from the long arm, and is also required for proper timing of HTP-1 and HTP-2 dissociation from the short arm. Phosphorylation of HIM-3 thus plays a role in establishing the identity of short and long arms, thereby contributing to the robustness of the two-step chromosome segregation.","journal":"PLoS Genetics","year":2020,"id":81572,"datarank":0.6019553836545826,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.19574785348925097,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.19574785348925097,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":9,"citers_with_citation_signal":9,"citers_with_endowment":9,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9522,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":263695,"name":"Chihiro Nakamura-Tabuchi","orcid":"0000-0002-9382-2657","position":1,"is_corresponding":false},{"id":260702,"name":"Xuan Li","orcid":"0000-0001-6319-3020","position":2,"is_corresponding":false},{"id":421869,"name":"Hendrik Boog","orcid":"0000-0002-3624-8345","position":3,"is_corresponding":false},{"id":421870,"name":"Madison K Lehmer","orcid":"0000-0003-3035-577X","position":4,"is_corresponding":false},{"id":421871,"name":"Scott Rosenberg","orcid":"0000-0001-8316-0613","position":5,"is_corresponding":false},{"id":421872,"name":"Consuelo Barroso","orcid":"0000-0002-0661-3460","position":6,"is_corresponding":false},{"id":421873,"name":"Enrique Martínez-Pérez","orcid":"0000-0001-5813-0383","position":7,"is_corresponding":false},{"id":106560,"name":"Kevin D. Corbett","orcid":"0000-0001-5854-2388","position":8,"is_corresponding":false},{"id":421874,"name":"Peter M. Carlton","orcid":"0000-0002-5320-6024","position":9,"is_corresponding":false},{"id":366633,"name":"Aya Sato","orcid":"0000-0003-0593-5238","position":0,"is_corresponding":true}],"reference_count":78,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T21:52:58.921847Z","pmid":"33175901","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}