{"doi":"10.1371/journal.pgen.1008609","title":"Octopamine neuron dependent aggression requires dVGLUT from dual-transmitting neurons","abstract":"Neuromodulators such as monoamines are often expressed in neurons that also release at least one fast-acting neurotransmitter. The release of a combination of transmitters provides both \"classical\" and \"modulatory\" signals that could produce diverse and/or complementary effects in associated circuits. Here, we establish that the majority of Drosophila octopamine (OA) neurons are also glutamatergic and identify the individual contributions of each neurotransmitter on sex-specific behaviors. Males without OA display low levels of aggression and high levels of inter-male courtship. Males deficient for dVGLUT solely in OA-glutamate neurons (OGNs) also exhibit a reduction in aggression, but without a concurrent increase in inter-male courtship. Within OGNs, a portion of VMAT and dVGLUT puncta differ in localization suggesting spatial differences in OA signaling. Our findings establish a previously undetermined role for dVGLUT in OA neurons and suggests that glutamate uncouples aggression from OA-dependent courtship-related behavior. These results indicate that dual neurotransmission can increase the efficacy of individual neurotransmitters while maintaining unique functions within a multi-functional social behavior neuronal network.","journal":"PLoS Genetics","year":2020,"id":59564,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":66,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9546,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":313881,"name":"Elizabeth Catudio Garrett","orcid":"0000-0002-5210-2688","position":1,"is_corresponding":false},{"id":313882,"name":"Hannah R. Morgan","orcid":"0000-0002-1370-9444","position":2,"is_corresponding":false},{"id":315846,"name":"Edmond D Brewer","orcid":null,"position":3,"is_corresponding":false},{"id":313883,"name":"Lucy A. Sirrs","orcid":"0000-0001-5031-3062","position":4,"is_corresponding":false},{"id":315847,"name":"Harold Shearin","orcid":null,"position":5,"is_corresponding":false},{"id":313884,"name":"Jessica L. Williams","orcid":"0000-0001-6924-7982","position":6,"is_corresponding":false},{"id":313885,"name":"Brian D. McCabe","orcid":"0000-0003-1620-0501","position":7,"is_corresponding":false},{"id":313886,"name":"R Steven Stowers","orcid":"0000-0002-1473-2478","position":8,"is_corresponding":false},{"id":313887,"name":"Sarah J. Certel","orcid":"0000-0003-2039-2657","position":9,"is_corresponding":false},{"id":313880,"name":"Lewis M. Sherer","orcid":"0000-0001-5875-1737","position":0,"is_corresponding":true}],"reference_count":100,"raw_metadata":null,"created_at":"2026-07-18T21:08:26.834549Z","pmid":"32097408","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}