{"doi":"10.1371/journal.pcbi.1012386","title":"Binomial models uncover biological variation during feature selection of droplet-based single-cell RNA sequencing","abstract":"Effective analysis of single-cell RNA sequencing (scRNA-seq) data requires a rigorous distinction between technical noise and biological variation. In this work, we propose a simple feature selection model, termed \"Differentially Distributed Genes\" or DDGs, where a binomial sampling process for each mRNA species produces a null model of technical variation. Using scRNA-seq data where cell identities have been established a priori, we find that the DDG model of biological variation outperforms existing methods. We demonstrate that DDGs distinguish a validated set of real biologically varying genes, minimize neighborhood distortion, and enable accurate partitioning of cells into their established cell-type groups.","journal":"PLoS Computational Biology","year":2024,"id":447427,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9525,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":988049,"name":"Timothy Hamilton","orcid":"0000-0002-8050-4860","position":1,"is_corresponding":false},{"id":988651,"name":"Gunalan Natesan","orcid":null,"position":2,"is_corresponding":false},{"id":1265055,"name":"Samuel D. Aragones","orcid":"0000-0002-5071-797X","position":3,"is_corresponding":false},{"id":162949,"name":"Eric J. Deeds","orcid":"0000-0002-2868-7495","position":4,"is_corresponding":false},{"id":988048,"name":"Breanne Sparta","orcid":"0000-0002-8255-1409","position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":null,"created_at":"2026-07-19T02:01:59.101070Z","pmid":"39241106","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}