{"doi":"10.1371/journal.pbio.3002874","title":"Structures of the mycobacterial MmpL4 and MmpL5 transporters provide insights into their role in siderophore export and iron acquisition","abstract":"The Mycobacterium tuberculosis (Mtb) pathogen, the causative agent of the airborne infection tuberculosis (TB), harbors a number of mycobacterial membrane protein large (MmpL) transporters. These membrane proteins can be separated into 2 distinct subclasses, where they perform important functional roles, and thus, are considered potential drug targets to combat TB. Previously, we reported both X-ray and cryo-EM structures of the MmpL3 transporter, providing high-resolution structural information for this subclass of the MmpL proteins. Currently, there is no structural information available for the subclass associated with MmpL4 and MmpL5, transporters that play a critical role in iron homeostasis of the bacterium. Here, we report cryo-EM structures of the M. smegmatis MmpL4 and MmpL5 transporters to resolutions of 2.95 Å and 3.00 Å, respectively. These structures allow us to propose a plausible pathway for siderophore translocation via these 2 transporters, an essential step for iron acquisition that enables the survival and replication of the mycobacterium.","journal":"PLoS Biology","year":2024,"id":425267,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.96,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":825086,"name":"Zhemin Zhang","orcid":"0000-0003-4110-3164","position":1,"is_corresponding":false},{"id":317328,"name":"Philip A. Klenotic","orcid":"0000-0001-8429-0145","position":2,"is_corresponding":false},{"id":1222888,"name":"William D. Gregor","orcid":"0009-0002-7722-614X","position":3,"is_corresponding":false},{"id":924645,"name":"Marios Tringides","orcid":"0000-0002-3800-2365","position":4,"is_corresponding":false},{"id":409994,"name":"Meng Cui","orcid":"0000-0002-3895-135X","position":5,"is_corresponding":false},{"id":417822,"name":"Georgiana E. Purdy","orcid":"0000-0002-0739-6867","position":6,"is_corresponding":false},{"id":308716,"name":"Edward Yu","orcid":"0000-0001-5912-1227","position":7,"is_corresponding":false},{"id":1222887,"name":"Rakesh Maharjan","orcid":"0000-0003-2217-3191","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-19T01:58:26.927596Z","pmid":"39423221","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}