{"doi":"10.1371/journal.pbio.3001284","title":"Mouse-adapted SARS-CoV-2 protects animals from lethal SARS-CoV challenge","abstract":"The emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has resulted in a pandemic causing significant damage to public health and the economy. Efforts to understand the mechanisms of Coronavirus Disease 2019 (COVID-19) have been hampered by the lack of robust mouse models. To overcome this barrier, we used a reverse genetic system to generate a mouse-adapted strain of SARS-CoV-2. Incorporating key mutations found in SARS-CoV-2 variants, this model recapitulates critical elements of human infection including viral replication in the lung, immune cell infiltration, and significant in vivo disease. Importantly, mouse adaptation of SARS-CoV-2 does not impair replication in human airway cells and maintains antigenicity similar to human SARS-CoV-2 strains. Coupled with the incorporation of mutations found in variants of concern, CMA3p20 offers several advantages over other mouse-adapted SARS-CoV-2 strains. Using this model, we demonstrate that SARS-CoV-2-infected mice are protected from lethal challenge with the original Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), suggesting immunity from heterologous Coronavirus (CoV) strains. Together, the results highlight the use of this mouse model for further study of SARS-CoV-2 infection and disease.","journal":"PLoS Biology","year":2021,"id":150924,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":88,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9428,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":550017,"name":"Michelle N. Vu","orcid":"0000-0002-3208-710X","position":1,"is_corresponding":false},{"id":105304,"name":"Bryan A. Johnson","orcid":"0000-0002-8999-1255","position":2,"is_corresponding":false},{"id":329379,"name":"Meredith E. Davis-Gardner","orcid":"0000-0002-6133-3613","position":3,"is_corresponding":false},{"id":106936,"name":"Abigail Vanderheiden","orcid":"0000-0003-1321-4510","position":4,"is_corresponding":false},{"id":105305,"name":"Kumari G. Lokugamage","orcid":null,"position":5,"is_corresponding":false},{"id":108475,"name":"Craig Schindewolf","orcid":"0000-0002-9804-2246","position":6,"is_corresponding":false},{"id":641735,"name":"Patricia A. Crocquet-Valdes","orcid":"0000-0002-2079-0076","position":7,"is_corresponding":false},{"id":641736,"name":"Rose M. Langsjoen","orcid":"0000-0001-9008-425X","position":8,"is_corresponding":false},{"id":105300,"name":"Jessica A. Plante","orcid":"0000-0002-4768-7458","position":9,"is_corresponding":false},{"id":105318,"name":"Kenneth S. Plante","orcid":"0000-0002-7485-3167","position":10,"is_corresponding":false},{"id":105319,"name":"Scott C. Weaver","orcid":"0000-0001-8016-8556","position":11,"is_corresponding":false},{"id":298531,"name":"Kari Debbink","orcid":"0000-0001-7242-7845","position":12,"is_corresponding":false},{"id":275508,"name":"Andrew Routh","orcid":"0000-0002-2874-5990","position":13,"is_corresponding":false},{"id":439576,"name":"David H. Walker","orcid":"0000-0002-3309-4360","position":14,"is_corresponding":false},{"id":106937,"name":"Mehul S. Suthar","orcid":"0000-0002-2686-8380","position":15,"is_corresponding":false},{"id":38215,"name":"Pei‐Yong Shi","orcid":"0000-0001-5553-1616","position":16,"is_corresponding":false},{"id":105317,"name":"Xuping Xie","orcid":"0000-0003-0918-016X","position":17,"is_corresponding":false},{"id":105316,"name":"Vineet D. Menachery","orcid":"0000-0001-8803-7606","position":18,"is_corresponding":false},{"id":105307,"name":"Antonio E. Muruato","orcid":null,"position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-18T23:43:11.293086Z","pmid":"34735434","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}