{"doi":"10.1371/journal.pbio.3000746","title":"Structure of TBC1D23 N-terminus reveals a novel role for rhodanese domain","abstract":"Members of the Tre2-Bub2-Cdc16 (TBC) family often function to regulate membrane trafficking and to control signaling transductions pathways. As a member of the TBC family, TBC1D23 is critical for endosome-to-Golgi cargo trafficking by serving as a bridge between Golgi-bound golgin-97/245 and the WASH/FAM21 complex on endosomal vesicles. However, the exact mechanisms by which TBC1D23 regulates cargo transport are poorly understood. Here, we present the crystal structure of the N-terminus of TBC1D23 (D23N), which consists of both the TBC and rhodanese domains. We show that the rhodanese domain is unlikely to be an active sulfurtransferase or phosphatase, despite containing a putative catalytic site. Instead, it packs against the TBC domain and forms part of the platform to interact with golgin-97/245. Using the zebrafish model, we show that impacting golgin-97/245-binding, but not the putative catalytic site, impairs neuronal growth and brain development. Altogether, our studies provide structural and functional insights into an essential protein that is required for organelle-specific trafficking and brain development.","journal":"PLoS Biology","year":2020,"id":103159,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9509,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":502955,"name":"Fan Yang","orcid":"0000-0002-4909-9094","position":1,"is_corresponding":false},{"id":477649,"name":"Zhe Liu","orcid":"0000-0003-4329-6799","position":2,"is_corresponding":false},{"id":502956,"name":"Jinrui Wang","orcid":"0000-0001-7373-2649","position":3,"is_corresponding":false},{"id":294306,"name":"Wenjie Huang","orcid":"0000-0003-3182-8516","position":4,"is_corresponding":false},{"id":502957,"name":"Wentong Meng","orcid":"0000-0002-9592-5668","position":5,"is_corresponding":false},{"id":290939,"name":"Daniel D. Billadeau","orcid":"0000-0002-2296-9547","position":6,"is_corresponding":false},{"id":290938,"name":"Qingxiang Sun","orcid":"0000-0002-9474-8882","position":7,"is_corresponding":false},{"id":477651,"name":"Xianming Mo","orcid":"0000-0003-3897-3126","position":8,"is_corresponding":false},{"id":290941,"name":"Da Jia","orcid":"0000-0002-2205-1998","position":9,"is_corresponding":false},{"id":503917,"name":"Dingdong Liu","orcid":null,"position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":null,"created_at":"2026-07-18T22:42:17.920911Z","pmid":"32453802","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}