{"doi":"10.1371/journal.pbio.3000631","title":"Mechanism of cargo recognition by retromer-linked SNX-BAR proteins","abstract":"Endocytic recycling of internalized transmembrane proteins is essential for many important physiological processes. Recent studies have revealed that retromer-related Sorting Nexin family (SNX)-Bin/Amphiphysin/Rvs (BAR) proteins can directly recognize cargoes like cation-independent mannose 6-phosphate receptor (CI-MPR) and Insulin-like growth factor 1 receptor (IGF1R); however, it remains poorly understood how SNX-BARs select specific cargo proteins and whether they recognize additional ligands. Here, we discovered that the binding between SNX-BARs and CI-MPR or IGF1R is mediated by the phox-homology (PX) domain of SNX5 or SNX6 and a bipartite motif, termed SNX-BAR-binding motif (SBM), in the cargoes. Using this motif, we identified over 70 putative SNX-BAR ligands, many of which play critical roles in apoptosis, cell adhesion, signal transduction, or metabolite homeostasis. Remarkably, SNX-BARs could cooperate with both SNX27 and retromer in the recycling of ligands encompassing the SBM, PDZ-binding motif, or both motifs. Overall, our studies establish that SNX-BARs function as a direct cargo-selecting module for a large set of transmembrane proteins transiting the endosome, in addition to their roles in phospholipid recognition and biogenesis of tubular structures.","journal":"PLoS Biology","year":2020,"id":56031,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":88,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.952,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":290931,"name":"Lin Zhao","orcid":"0000-0002-6056-8787","position":1,"is_corresponding":false},{"id":290932,"name":"Wankun Deng","orcid":"0000-0002-5052-9151","position":2,"is_corresponding":false},{"id":290933,"name":"Hongbin Sun","orcid":"0000-0002-7712-0655","position":3,"is_corresponding":false},{"id":290934,"name":"Xuedong Zhou","orcid":"0000-0002-9319-7302","position":4,"is_corresponding":false},{"id":290935,"name":"Lejiao Mao","orcid":"0000-0001-6044-4971","position":5,"is_corresponding":false},{"id":290936,"name":"Wenfeng Hu","orcid":"0000-0002-2571-3889","position":6,"is_corresponding":false},{"id":290937,"name":"Xiaofei Shen","orcid":"0000-0001-6862-634X","position":7,"is_corresponding":false},{"id":290938,"name":"Qingxiang Sun","orcid":"0000-0002-9474-8882","position":8,"is_corresponding":false},{"id":290939,"name":"Daniel D. Billadeau","orcid":"0000-0002-2296-9547","position":9,"is_corresponding":false},{"id":290940,"name":"Yu Xue","orcid":"0000-0002-9403-6869","position":10,"is_corresponding":false},{"id":290941,"name":"Da Jia","orcid":"0000-0002-2205-1998","position":11,"is_corresponding":false},{"id":293318,"name":"Xin Yong","orcid":null,"position":0,"is_corresponding":true}],"reference_count":62,"raw_metadata":null,"created_at":"2026-07-18T21:05:49.581291Z","pmid":"32150533","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}