{"doi":"10.1364/optica.392805","title":"Sparse decomposition light-field microscopy for high speed imaging of neuronal activity","abstract":"One of the major challenges in large scale optical imaging of neuronal activity is to simultaneously achieve sufficient temporal and spatial resolution across a large volume. Here, we introduce sparse decomposition light-field microscopy (SDLFM), a computational imaging technique based on light-field microscopy (LFM) that takes algorithmic advantage of the high temporal resolution of LFM and the inherent temporal sparsity of spikes to improve effective spatial resolution and signal-to-noise ratios (SNRs). With increased effective spatial resolution and SNRs, neuronal activity at the single-cell level can be recovered over a large volume. We demonstrate the single-cell imaging capability of SDLFM with in vivo imaging of neuronal activity of whole brains of larval zebrafish with estimated lateral and axial resolutions of <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" display=\"inline\"> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mo>∼</mml:mo> </mml:mrow> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mn>3.5</mml:mn> </mml:mrow> <mml:mspace width=\"thickmathspace\"/> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mtext>µ</mml:mtext> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mi mathvariant=\"normal\">m</mml:mi> </mml:mrow> </mml:mrow> </mml:mrow> </mml:math> and <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" display=\"inline\"> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mo>∼</mml:mo> </mml:mrow> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mn>7.4</mml:mn> </mml:mrow> <mml:mspace width=\"thickmathspace\"/> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mtext>µ</mml:mtext> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mi mathvariant=\"normal\">m</mml:mi> </mml:mrow> </mml:mrow> </mml:mrow> </mml:math> , respectively, acquired at volumetric imaging rates up to 50 Hz. We also show that SDLFM increases the quality of neural imaging in adult fruit flies.","journal":"Optica","year":2020,"id":58211,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":74,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9606,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":304873,"name":"Zeguan Wang","orcid":"0000-0001-9149-3389","position":1,"is_corresponding":false},{"id":94533,"name":"Nikita Pak","orcid":"0000-0002-7288-0479","position":2,"is_corresponding":false},{"id":257211,"name":"Demian Park","orcid":null,"position":3,"is_corresponding":false},{"id":304874,"name":"Peilun Dai","orcid":"0000-0002-1680-0526","position":4,"is_corresponding":false},{"id":306452,"name":"Jeong Seuk Kang","orcid":null,"position":5,"is_corresponding":false},{"id":94612,"name":"Ho‐Jun Suk","orcid":"0000-0002-0899-6709","position":6,"is_corresponding":false},{"id":54475,"name":"Panagiotis Symvoulidis","orcid":"0000-0002-1396-3426","position":7,"is_corresponding":false},{"id":264906,"name":"Burcu Guner‐Ataman","orcid":null,"position":8,"is_corresponding":false},{"id":304875,"name":"Kai Wang","orcid":"0000-0001-7858-944X","position":9,"is_corresponding":false},{"id":11617,"name":"Edward S. Boyden","orcid":"0000-0002-0419-3351","position":10,"is_corresponding":false},{"id":304872,"name":"Young‐Gyu Yoon","orcid":"0000-0002-5660-049X","position":0,"is_corresponding":true}],"reference_count":38,"raw_metadata":null,"created_at":"2026-07-18T21:07:11.345501Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}