{"doi":"10.1364/ol.521591","title":"Axially swept open-top light-sheet microscopy for densely labeled clinical specimens","abstract":"Open-top light-sheet (OTLS) microscopy offers rapid 3D imaging of large optically cleared specimens. This enables nondestructive 3D pathology, which provides key advantages over conventional slide-based histology including comprehensive sampling without tissue sectioning/destruction and visualization of diagnostically important 3D structures. With 3D pathology, clinical specimens are often labeled with small-molecule stains that broadly target nucleic acids and proteins, mimicking conventional hematoxylin and eosin (H&E) dyes. Tight optical sectioning helps to minimize out-of-focus fluorescence for high-contrast imaging in these densely labeled tissues but has been challenging to achieve in OTLS systems due to trade-offs between optical sectioning and field of view. Here we present an OTLS microscope with voice-coil-based axial sweeping to circumvent this trade-off, achieving 2 µm axial resolution over a 750 × 375 µm field of view. We implement our design in a non-orthogonal dual-objective (NODO) architecture, which enables a 10-mm working distance with minimal sensitivity to refractive index mismatches, for high-contrast 3D imaging of clinical specimens.","journal":"Optics Letters","year":2024,"id":440477,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.959,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":560155,"name":"Lindsey A. Barner","orcid":"0000-0002-9156-0024","position":1,"is_corresponding":false},{"id":1164651,"name":"Elena Baraznenok","orcid":null,"position":2,"is_corresponding":false},{"id":1051528,"name":"Lydia Lan","orcid":null,"position":3,"is_corresponding":false},{"id":258186,"name":"Chetan Poudel","orcid":"0000-0002-8512-9238","position":4,"is_corresponding":false},{"id":451833,"name":"David Brenes","orcid":"0000-0003-1969-5386","position":5,"is_corresponding":false},{"id":323421,"name":"Robert Serafin","orcid":"0000-0002-6646-353X","position":6,"is_corresponding":false},{"id":360871,"name":"Lawrence D. True","orcid":"0000-0001-5682-1228","position":7,"is_corresponding":false},{"id":279206,"name":"Joshua C. Vaughan","orcid":"0000-0002-6550-8935","position":8,"is_corresponding":false},{"id":279202,"name":"Adam K. Glaser","orcid":"0000-0003-3558-8994","position":9,"is_corresponding":false},{"id":20776,"name":"Jonathan Liu","orcid":"0000-0001-5650-3086","position":10,"is_corresponding":false},{"id":353034,"name":"Kevin W. Bishop","orcid":"0000-0001-8722-6464","position":0,"is_corresponding":true}],"reference_count":21,"raw_metadata":null,"created_at":"2026-07-19T02:00:57.233748Z","pmid":"38950270","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}