{"doi":"10.1364/ol.493824","title":"Dual-modality imaging system for monitoring human heart organoids beating in vitro","abstract":"To reveal the three-dimensional microstructure and calcium dynamics of human heart organoids (hHOs), we developed a dual-modality imaging system combining the advantages of optical coherence tomography (OCT) and fluorescence microscopy. OCT provides high-resolution volumetric structural information, while fluorescence imaging indicates the electrophysiology of the hHOs' beating behavior. We verified that concurrent OCT motion mode (M-mode) and calcium imaging retrieved the same beating pattern from the heart organoids. We further applied dynamic contrast OCT (DyC-OCT) analysis to strengthen the verification and localize the beating clusters inside the hHOs. This imaging platform provides a powerful tool for studying and assessing hHOs in vitro, with potential applications in disease modeling and drug screening.","journal":"Optics Letters","year":2023,"id":329461,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9545,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":246654,"name":"Chao Ren","orcid":"0000-0002-5418-3354","position":1,"is_corresponding":false},{"id":1052155,"name":"Fei Wang","orcid":"0000-0003-1766-288X","position":2,"is_corresponding":false},{"id":1019532,"name":"Kibeom Park","orcid":"0000-0001-6344-5427","position":3,"is_corresponding":false},{"id":616014,"name":"Brett Volmert","orcid":"0000-0002-5209-4833","position":4,"is_corresponding":false},{"id":616019,"name":"Aitor Aguirre","orcid":"0000-0002-1383-2792","position":5,"is_corresponding":false},{"id":402103,"name":"Chao Zhou","orcid":"0000-0002-8679-3413","position":6,"is_corresponding":false},{"id":994500,"name":"Senyue Hao","orcid":"0009-0005-0860-7629","position":0,"is_corresponding":true}],"reference_count":27,"raw_metadata":null,"created_at":"2026-07-19T01:09:01.509601Z","pmid":"37527085","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}