{"doi":"10.1364/ol.391824","title":"High-speed wide-field multi-parametric photoacoustic microscopy","abstract":"Capable of imaging blood perfusion, oxygenation, and flow simultaneously at the microscopic level, multi-parametric photoacoustic microscopy (PAM) has quickly emerged as a powerful tool for studying hemodynamic and metabolic changes due to physiological stimulations or pathological processes. However, the low scanning speed poised by the correlation-based blood flow measurement impedes its application in studying rapid microvascular responses. To address this challenge, we have developed a new, to the best of our knowledge, multi-parametric PAM system. By extending the optical scanning range with a cylindrically focused ultrasonic transducer (focal zone, <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" display=\"inline\"> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mn>76</mml:mn> </mml:mrow> <mml:mspace width=\"thickmathspace\"/> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mtext>µ</mml:mtext> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mi mathvariant=\"normal\">m</mml:mi> </mml:mrow> </mml:mrow> <mml:mo>×</mml:mo> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mn>4.5</mml:mn> </mml:mrow> <mml:mspace width=\"thickmathspace\"/> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mi mathvariant=\"normal\">m</mml:mi> <mml:mi mathvariant=\"normal\">m</mml:mi> </mml:mrow> </mml:math> ) for simultaneous acquisition of 500 B-scans, the new system is 112 times faster than our previous multi-parametric system that uses a spherically focused transducer (focal diameter, 40 µm) and enables high-resolution imaging of blood perfusion, oxygenation, and flow over an area of <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" display=\"inline\"> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mn>4.5</mml:mn> </mml:mrow> <mml:mo>×</mml:mo> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mn>1</mml:mn> </mml:mrow> <mml:mspace width=\"thickmathspace\"/> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:msup> <mml:mrow class=\"MJX-TeXAtom-ORD\"> <mml:mi mathvariant=\"normal\">m</mml:mi> <mml:mi mathvariant=\"normal\">m</mml:mi> </mml:mrow> <mml:mn>2</mml:mn> </mml:msup> </mml:mrow> </mml:math> at a frame rate of 1 Hz. We have demonstrated the feasibility of this system in the living mouse ear. Further development of this system into reflection mode will enable real-time cortex-wide imaging of hemodynamics and metabolism in the mouse brain.","journal":"Optics Letters","year":2020,"id":95823,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":35,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9554,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":476306,"name":"Youwei Bao","orcid":null,"position":1,"is_corresponding":false},{"id":404259,"name":"Ruimin Chen","orcid":"0000-0002-5338-359X","position":2,"is_corresponding":false},{"id":266837,"name":"Qifa Zhou","orcid":"0000-0003-1527-3020","position":3,"is_corresponding":false},{"id":475501,"name":"Song Hu","orcid":"0000-0002-5760-8012","position":4,"is_corresponding":false},{"id":475500,"name":"Fenghe Zhong","orcid":"0000-0002-1281-9465","position":0,"is_corresponding":true}],"reference_count":15,"raw_metadata":null,"created_at":"2026-07-18T22:33:57.534608Z","pmid":"32412459","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}