{"doi":"10.1364/ol.381571","title":"Two-photon microscope using a fiber-based approach for supercontinuum generation and light delivery to a small-footprint optical head","abstract":"In this Letter, we report a low-cost, portable, two-photon excitation fluorescence microscopy imager that uses a fiber-based approach for both femtosecond supercontinuum (SC) generation and light delivery to the optical head. The SC generation is based on a tapered polarization-maintaining photonic crystal fiber that uses pre-chirped femtosecond narrowband pulses to generate a coherent SC spectrum with a bandwidth of approximately 300 nm. Using this approach, high-power, near-transform-limited, wavelength-selectable SC pulses are generated and directly delivered to the imaging optical head. Preliminary testing of this imager on brain slices is presented, demonstrating a high signal-to-noise ratio and sub-cellular imaging capabilities to a depth of approximately 200 µm. These results demonstrate the suitability of the technology for ex vivo and potentially in vivo cellular-level biomedical imaging applications.","journal":"Optics Letters","year":2020,"id":106076,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9603,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":431244,"name":"Gopi Maguluri","orcid":null,"position":1,"is_corresponding":false},{"id":512823,"name":"R. Daniel Ferguson","orcid":null,"position":2,"is_corresponding":false},{"id":417936,"name":"Haohua Tu","orcid":"0000-0003-3116-0149","position":3,"is_corresponding":false},{"id":512824,"name":"Kush Paul","orcid":null,"position":4,"is_corresponding":false},{"id":366688,"name":"Stephen A. Boppart","orcid":"0000-0002-9386-5630","position":5,"is_corresponding":false},{"id":417937,"name":"Daniel A. Llano","orcid":"0000-0003-0933-1837","position":6,"is_corresponding":false},{"id":430214,"name":"Nicusor Iftimia","orcid":"0000-0002-7713-6972","position":7,"is_corresponding":false},{"id":397808,"name":"Youbo Zhao","orcid":"0000-0003-1667-2373","position":0,"is_corresponding":true}],"reference_count":27,"raw_metadata":null,"created_at":"2026-07-18T23:12:23.770754Z","pmid":"32058502","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}