{"doi":"10.1364/oe.404869","title":"Volumetric chemical imaging in vivo by a remote-focusing stimulated Raman scattering microscope","abstract":"Operable under ambient light and providing chemical selectivity, stimulated Raman scattering (SRS) microscopy opens a new window for imaging molecular events on a human subject, such as filtration of topical drugs through the skin. A typical approach for volumetric SRS imaging is through piezo scanning of an objective lens, which often disturbs the sample and offers a low axial scan rate. To address these challenges, we have developed a deformable mirror-based remote-focusing SRS microscope, which not only enables high-quality volumetric chemical imaging without mechanical scanning of the objective but also corrects the system aberrations simultaneously. Using the remote-focusing SRS microscope, we performed volumetric chemical imaging of living cells and captured in real time the dynamic diffusion of topical chemicals into human sweat pores.","journal":"Optics Express","year":2020,"id":99448,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9594,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":489253,"name":"Hongli Ni","orcid":"0000-0003-4323-1493","position":1,"is_corresponding":false},{"id":489772,"name":"Huate Li","orcid":null,"position":2,"is_corresponding":false},{"id":489254,"name":"Nicholas A. Vickers","orcid":"0000-0002-2293-9671","position":3,"is_corresponding":false},{"id":273340,"name":"Yuying Tan","orcid":"0000-0003-2536-4778","position":4,"is_corresponding":false},{"id":489773,"name":"Ruyi Gong","orcid":null,"position":5,"is_corresponding":false},{"id":489255,"name":"Thomas G. Bifano","orcid":"0000-0003-4952-2515","position":6,"is_corresponding":false},{"id":266496,"name":"Ji‐Xin Cheng","orcid":"0000-0002-5607-6683","position":7,"is_corresponding":false},{"id":489252,"name":"Peng Lin","orcid":"0000-0002-2817-0744","position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":null,"created_at":"2026-07-18T22:37:35.547042Z","pmid":"33114904","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}