{"doi":"10.1364/oe.20.004957","title":"PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking","abstract":null,"journal":"Optics Express","year":2012,"id":678947,"datarank":0.7649799641736299,"base_score":5.099866427824199,"endowment":5.099866427824199,"self_citation_contribution":0.7649799641736299,"citation_network_contribution":0.0,"self_endowment_contribution":0.7649799641736299,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":163,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":2,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1773920,"name":"Mohamed El Beheiry","orcid":null,"position":1,"is_corresponding":false},{"id":1773921,"name":"Jordi Andilla","orcid":null,"position":2,"is_corresponding":false},{"id":1773922,"name":"Daniel Ciepielewski","orcid":null,"position":3,"is_corresponding":false},{"id":109376,"name":"Xavier Darzacq","orcid":"0000-0003-2537-8395","position":4,"is_corresponding":false},{"id":244946,"name":"Maxime Dahan","orcid":"0000-0002-4943-2491","position":5,"is_corresponding":false},{"id":1773919,"name":"Ignacio Izeddin","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking","abstract":"We present a novel approach for three-dimensional localization of single molecules using adaptive optics. A 52-actuator deformable mirror is used to both correct aberrations and induce two-dimensional astigmatism in the point-spread-function. The dependence of the z-localization precision on the degree of astigmatism is discussed. We achieve a z-localization precision of 40 nm for fluorescent proteins and 20 nm for fluorescent dyes, over an axial depth of ~800 nm. We illustrate the capabilities of our approach for three-dimensional high-resolution microscopy with super-resolution images of actin filaments in fixed cells and single-molecule tracking of quantum-dot labeled transmembrane proteins in live HeLa cells.","is_dataset_classified":null,"base_score":5.099866427824199,"endowment":5.099866427824199,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22418300","pmcid":null,"openalex_id":"https://openalex.org/W2090227459","authors":[],"funders":[],"total_grants":0,"fwci":14.784,"citation_percentile":0.99147324,"influential_citations":7,"citation_trend":[{"year":2012,"count":6},{"year":2013,"count":7},{"year":2014,"count":20},{"year":2015,"count":25},{"year":2016,"count":10},{"year":2017,"count":12},{"year":2018,"count":21},{"year":2019,"count":6},{"year":2020,"count":5},{"year":2021,"count":12},{"year":2022,"count":12},{"year":2023,"count":7},{"year":2024,"count":14},{"year":2025,"count":5},{"year":2026,"count":1}],"oa_status":"gold","license":"https://doi.org/10.1364/OA_License_v1#VOR-OA","oa_locations":[{"url":"https://doi.org/10.1364/oe.20.004957","host_type":"journal"},{"url":"https://doi.org/10.1364/oe.20.004957","host_type":"GOLD"},{"url":"https://doi.org/10.1364/oe.20.004957","host_type":"publisher"},{"url":"https://www.osapublishing.org/viewmedia.cfm?URI=oe-20-5-4957&seq=0","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22418300","host_type":"repository"}],"fields_of_study":["Advanced Fluorescence Microscopy Techniques","Advanced Electron Microscopy Techniques and Applications","Optical Coherence Tomography Applications","Physics","Medicine","Engineering"],"mesh_terms":["Equipment Design","HeLa Cells","Humans","Image Enhancement","Lenses","Microscopy","Proteins","Sensitivity and Specificity","Reproducibility of Results","Equipment Failure Analysis","Hela Cells"],"keywords":["Optics","Point spread function","Adaptive optics","Deformable mirror","Super-resolution microscopy","Microscopy","Tracking (education)","Resolution (logic)","Image resolution","Physics","Fluorescence","Astigmatism","Fluorescence microscope","Materials science","Computer science"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.c.3764180","title":"PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking","publisher":"Figshare","resource_type":"Collection"},{"doi":"10.6084/m9.figshare.c.3764180.v1","title":"PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking","publisher":"Figshare","resource_type":"Collection"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T11:53:42.337928Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}