{"doi":"10.1364/boe.561992","title":"Integrated visible-light optical coherence tomography and fluorescence scanning laser ophthalmoscopy to image retinal ganglion cell axons","abstract":"Retinal ganglion cell (RGC) soma and axonal damage is a hallmark of optic neuropathies. Visible-light OCT fibergraphy (vis-OCTF) enables non-invasive imaging and quantitative assessment of individual RGC axon bundles; however, validating vis-OCTF using confocal fluorescence imaging of flat-mounted postmortem retina is less accurate due to structural alterations caused by flat-mount preparation and cannot be performed longitudinally. For in vivo vis-OCTF validation, we developed an integrated visible-light optical coherence tomography (vis-OCT) and fluorescence scanning laser ophthalmoscopy (SLO) system. The vis-OCT had a 100 nm bandwidth with a center wavelength of 560 nm, offering an axial resolution of 1.3 µm in the retina. The lateral resolutions of vis-OCT and SLO were 4 µm and 3.5 µm, respectively. In the transgenic Eno2-YFP mice, we showed that vis-OCTF and SLO provide consistent RGC axon bundle imaging results. Measuring 30 axon bundle widths from six mice yielded a Pearson correlation coefficient of 0.991 between SLO and vis-OCTF. Thus, the combined SLO and vis-OCT can potentially achieve multimodal longitudinal in vivo studies of RGC pathologies.","journal":"Biomedical Optics Express","year":2025,"id":539603,"datarank":0.21885480423235312,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.010910650064369509,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.010910650064369509,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":1,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9564,"is_data_producer":true,"deposit_databanks":{"figshare":["10.6084/m9.figshare.29312957"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":998222,"name":"Weijia Fan","orcid":"0009-0002-8173-0760","position":1,"is_corresponding":false},{"id":409027,"name":"Roman V. Kuranov","orcid":"0000-0002-9668-2960","position":2,"is_corresponding":false},{"id":563465,"name":"Junghun Kweon","orcid":"0000-0002-7538-7663","position":3,"is_corresponding":false},{"id":1384623,"name":"Raymond Atkinson","orcid":null,"position":4,"is_corresponding":false},{"id":1235842,"name":"Yong Hu","orcid":"0000-0002-7721-9268","position":5,"is_corresponding":false},{"id":456664,"name":"Cheng Sun","orcid":"0000-0002-2744-0896","position":6,"is_corresponding":false},{"id":409028,"name":"Hao F. Zhang","orcid":"0000-0001-5089-1196","position":7,"is_corresponding":false},{"id":1426654,"name":"Tianyi Xu","orcid":"0000-0001-6697-2492","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":null,"created_at":"2026-07-19T02:52:30.048313Z","pmid":"40677831","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}