{"doi":"10.1364/boe.534399","title":"Video-rate two-photon microendoscopy using second harmonic resonance fiber scanning","abstract":"We present a 2.5-mm-diameter resonant fiber scanning two-photon microendoscope with a 30-mm long forward-viewing rigid probe tip that enables video-rate imaging (20 Hz frame rate) suitable for hand-held imaging of tissues without motion artifacts. Higher-order harmonic oscillation scanning techniques are developed to significantly increase the frame rate compared to prior published fiber scanning microendoscopy designs while maintaining the field-of-view (∼125 µm), the optical resolution (1.2 µm lateral and 10.9 µm axial resolution, full width at half maximum), and the spatial sampling (1250 circumferential pixels per spiral × 20 radial pixels over the diameter; 210 spirals per frame, ∼4 spiral samples per resolvable pixel) compared to a traditional scan using the fundamental resonance. 3D printed mounts were created to reduce the cost and simplify the fabrication for the fiber scanner without compromising performance or stability (<0.3 µm drift over 84 hours). A custom long-wavelength (∼1.08 µm) femtosecond fiber laser is coupled into several meters of fiber to realize a flexible, hand-held device for long-wavelength multiphoton microendoscopy.","journal":"Biomedical Optics Express","year":2024,"id":481453,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":1,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9582,"is_data_producer":true,"deposit_databanks":{"figshare":["10.6084/m9.figshare.26156380"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1321499,"name":"Guillaume Ducourthial","orcid":"0000-0002-8252-3707","position":1,"is_corresponding":false},{"id":1321859,"name":"Taresh Sharan","orcid":null,"position":2,"is_corresponding":false},{"id":1298023,"name":"Arvind Mohan","orcid":"0000-0003-0419-4212","position":3,"is_corresponding":false},{"id":1321860,"name":"Jiasen Hou","orcid":null,"position":4,"is_corresponding":false},{"id":955811,"name":"Sudip Timilsina","orcid":"0009-0002-5789-2073","position":5,"is_corresponding":false},{"id":341980,"name":"Rongguang Liang","orcid":"0000-0003-2792-3457","position":6,"is_corresponding":false},{"id":1321861,"name":"Frederic Louradour","orcid":null,"position":7,"is_corresponding":false},{"id":397926,"name":"Bryan Q. Spring","orcid":"0000-0001-7332-7707","position":8,"is_corresponding":false},{"id":1321498,"name":"Kai Zhang","orcid":"0000-0002-5954-5764","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":null,"created_at":"2026-07-19T02:07:10.067421Z","pmid":"39553867","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}