{"doi":"10.1364/boe.5.001378","title":"Hyperspectral and differential CARS microscopy for quantitative chemical imaging in human adipocytes","abstract":null,"journal":"Biomedical Optics Express","year":2014,"id":607079,"datarank":0.6038027536102726,"base_score":4.02535169073515,"endowment":4.02535169073515,"self_citation_contribution":0.6038027536102726,"citation_network_contribution":0.0,"self_endowment_contribution":0.6038027536102726,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1558702,"name":"Iestyn Pope","orcid":null,"position":1,"is_corresponding":false},{"id":1558703,"name":"Francesco Masia","orcid":null,"position":2,"is_corresponding":false},{"id":127680,"name":"Peter Watson","orcid":null,"position":3,"is_corresponding":false},{"id":1558704,"name":"Wolfgang Langbein","orcid":null,"position":4,"is_corresponding":false},{"id":755863,"name":"Paola Borri","orcid":"0000-0002-7873-3314","position":5,"is_corresponding":false},{"id":1047182,"name":"Claudia Di Napoli","orcid":"0000-0001-7460-7137","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Hyperspectral and differential CARS microscopy for quantitative chemical imaging in human adipocytes","abstract":"In this work, we demonstrate the applicability of coherent anti-Stokes Raman scattering (CARS) micro-spectroscopy for quantitative chemical imaging of saturated and unsaturated lipids in human stem-cell derived adipocytes. We compare dual-frequency/differential CARS (D-CARS), which enables rapid imaging and simple data analysis, with broadband hyperspectral CARS microscopy analyzed using an unsupervised phase-retrieval and factorization method recently developed by us for quantitative chemical image analysis. Measurements were taken in the vibrational fingerprint region (1200-2000/cm) and in the CH stretch region (2600-3300/cm) using a home-built CARS set-up which enables hyperspectral imaging with 10/cm resolution via spectral focussing from a single broadband 5 fs Ti:Sa laser source. Through a ratiometric analysis, both D-CARS and phase-retrieved hyperspectral CARS determine the concentration of unsaturated lipids with comparable accuracy in the fingerprint region, while in the CH stretch region D-CARS provides only a qualitative contrast owing to its non-linear behavior. When analyzing hyperspectral CARS images using the blind factorization into susceptibilities and concentrations of chemical components recently demonstrated by us, we are able to determine vol:vol concentrations of different lipid components and spatially resolve inhomogeneities in lipid composition with superior accuracy compared to state-of-the art ratiometric methods.","is_dataset_classified":null,"base_score":4.02535169073515,"endowment":4.02535169073515,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24877002","pmcid":"PMC4026891","openalex_id":"https://openalex.org/W2059675262","authors":[],"funders":[{"funder_name":"Biotechnology and Biological Sciences Research Council (BBSRC)","grant_id":"BB/H006575/1","title":"Multiphoton microscopy of lipid-protein dynamics in living cells using correlative Coherent Antistokes Raman Scattering and Two-Photon Fluorescence"},{"funder_name":"Engineering and Physical Sciences Research Council (EPSRC)","grant_id":"EP/H045848/1","title":"PROBING THE MECHANICAL CONTROL OF STEM CELL FATE THROUGH THE DEVELOPMENT OF NOVEL, NON-INVASIVE IMAGING TECHNOLOGIES"},{"funder_name":"Engineering and Physical Sciences Research Council (EPSRC)","grant_id":"EP/I005072/1","title":"Shedding new light on cells with coherent multiphoton nanoscopy"},{"funder_name":"Engineering and Physical Sciences Research Council (EPSRC)","grant_id":"EP/I016260/1","title":"Physics-Life Sciences Interface C-DIP Fellowship Fund, EPSRC Leadership Fellowship Dr. Paola Borri"},{"funder_name":"European Commission","grant_id":"276807","title":"Probing the Fate of Stem Cells through the Development of a Multiplex Coherent Anti-Stokes Raman Scattering Imaging Technique"}],"total_grants":5,"fwci":6.6476,"citation_percentile":0.96263706,"influential_citations":0,"citation_trend":[{"year":2014,"count":2},{"year":2015,"count":6},{"year":2016,"count":6},{"year":2017,"count":3},{"year":2018,"count":7},{"year":2019,"count":5},{"year":2020,"count":6},{"year":2021,"count":5},{"year":2022,"count":4},{"year":2023,"count":5},{"year":2024,"count":3},{"year":2025,"count":1},{"year":2026,"count":2}],"oa_status":"gold","license":"https://doi.org/10.1364/OA_License_v1#VOR-OA","oa_locations":[{"url":"https://doi.org/10.1364/boe.5.001378","host_type":"journal"},{"url":"https://doi.org/10.1364/boe.5.001378","host_type":"publisher"},{"url":"https://www.osapublishing.org/viewmedia.cfm?URI=boe-5-5-1378&seq=0","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24877002","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4026891","host_type":"repository"},{"url":"https://dx.doi.org/10.1364/boe.5.001378","host_type":""},{"url":"http://dx.doi.org/10.1364/BOE.5.001378","host_type":""}],"fields_of_study":["Spectroscopy Techniques in Biomedical and Chemical Research","Spectroscopy and Chemometric Analyses","Mass Spectrometry Techniques and Applications","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Hyperspectral imaging","Microscopy","Chemical imaging","Optics","Remote sensing","Computer science","Artificial intelligence","Physics","Geology","(180.5655) Raman Microscopy","(300.6230) Spectroscopy, Coherent Anti-stokes Raman Scattering"],"sdg_mappings":[{"sdg_number":6,"sdg_label":"6. 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