{"doi":"10.1364/boe.448473","title":"Evaluation of resonant scanning as a high-speed imaging technique for two-photon imaging of cortical vasculature","abstract":"We demonstrate a simple, low-cost two-photon microscope design with both galvo-galvo and resonant-galvo scanning capabilities. We quantify and compare the signal-to-noise ratios and imaging speeds of the galvo-galvo and resonant-galvo scanning modes when used for murine neurovascular imaging. The two scanning modes perform as expected under shot-noise limited detection and are found to achieve comparable signal-to-noise ratios. Resonant-galvo scanning is capable of reaching desired signal-to-noise ratios using less acquisition time when higher excitation power can be used. Given equal excitation power and total pixel dwell time between the two methods, galvo-galvo scanning outperforms resonant-galvo scanning in image quality when detection deviates from being shot-noise limited.","journal":"Biomedical Optics Express","year":2022,"id":251779,"datarank":0.8582476566799998,"base_score":3.2188758248682006,"endowment":3.2188758248682006,"self_citation_contribution":0.48283137373023016,"citation_network_contribution":0.37541628294976964,"self_endowment_contribution":0.48283137373023016,"citer_contribution":0.37541628294976964,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":13,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9518,"is_data_producer":true,"deposit_databanks":{"figshare":["10.6084/m9.figshare.19064720"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":895216,"name":"Shaun A. Engelmann","orcid":"0000-0002-2410-896X","position":1,"is_corresponding":false},{"id":421476,"name":"Samuel A. Mihelic","orcid":null,"position":2,"is_corresponding":false},{"id":895740,"name":"Alankrit Tomar","orcid":null,"position":3,"is_corresponding":false},{"id":570360,"name":"Ahmed M. Hassan","orcid":"0009-0002-7989-0466","position":4,"is_corresponding":false},{"id":288013,"name":"Andrew K. Dunn","orcid":"0000-0001-6500-5783","position":5,"is_corresponding":false},{"id":895215,"name":"Annie Zhou","orcid":"0000-0003-0479-2941","position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":null,"created_at":"2026-07-19T00:24:41.993282Z","pmid":"35414984","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}