{"doi":"10.1364/boe.441118","title":"Mammalian complex III heme dynamics studied with pump-probe spectroscopy and red light illuminations","abstract":"The electronic or molecular mechanisms that initiate photobiomodulation (PBM) in cells are not yet fully understood. The porcine complex III (C-III) of the electron transport chain was characterized with transient absorption spectroscopy (TAS). We then applied our recently developed continuous wave laser coupled TAS procedure (CW-TAS) to investigate the effect of red light irradiances on the heme dynamics of C-III in its c 1 reduced state. The time constants were found to be 3.3 ± 0.3 ps for vibrational cooling of the oxidized state and 4.9 ± 0.4 ps for rebinding of the photodissociated axial ligand of the c 1 reduced state. The analysis of the CW-TAS procedure yielded no significant changes in the C-III heme dynamics. We rule out the possibility of 635 nm CW light at 4.7 mW/cm 2 inducing a PBM effect on the heme dynamic of C-III, specifically with the photodissociation of its axial ligand.","journal":"Biomedical Optics Express","year":2021,"id":208573,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9478,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":764413,"name":"Samantha Powell","orcid":"0000-0003-2670-0024","position":1,"is_corresponding":false},{"id":795206,"name":"John M. Rickman","orcid":"0000-0002-1188-8805","position":2,"is_corresponding":false},{"id":765077,"name":"Nathaniel J. Pope","orcid":null,"position":3,"is_corresponding":false},{"id":764412,"name":"Gary D. Noojin","orcid":"0009-0006-8311-571X","position":4,"is_corresponding":false},{"id":367274,"name":"Marlan O. Scully","orcid":"0000-0001-6397-4927","position":5,"is_corresponding":false},{"id":764414,"name":"Michael L. Denton","orcid":"0000-0003-3421-4085","position":6,"is_corresponding":false},{"id":360898,"name":"Vladislav V. Yakovlev","orcid":"0000-0002-4557-1013","position":7,"is_corresponding":false},{"id":795205,"name":"Sean P. O’Connor","orcid":"0000-0002-0314-8428","position":0,"is_corresponding":true}],"reference_count":22,"raw_metadata":null,"created_at":"2026-07-18T23:51:57.582057Z","pmid":"34858701","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}