{"doi":"10.1364/boe.393931","title":"Extended depth of focus multiphoton microscopy via incoherent pulse splitting","abstract":"We present a beam splitter mask that can be easily added to a multiphoton raster scanning microscope to extend the depth of focus five-fold at a small loss in lateral resolution. The method is designed for ultrafast laser pulses or other light-sources featuring a low coherence length. In contrast to other methods of focus extension, our approach uniquely combines low complexity, high light-throughput and multicolor capability. We characterize the point spread function in a two-photon microscope and demonstrate fluorescence imaging of GFP labeled neurons in fixed brain samples as imaged with conventional and extended depth of focus two-photon microscopy.","journal":"Biomedical Optics Express","year":2020,"id":70459,"datarank":1.054934781352942,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":0.6055749403198433,"self_endowment_contribution":0.4493598410330987,"citer_contribution":0.6055749403198433,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":18,"citers_with_citation_signal":14,"citers_with_endowment":14,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9581,"is_data_producer":true,"deposit_databanks":{"figshare":["10.6084/m9.figshare.12049020"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":353380,"name":"Tonmoy Chakraborty","orcid":"0000-0001-7956-1932","position":1,"is_corresponding":false},{"id":335040,"name":"Stephan Daetwyler","orcid":"0000-0002-7444-4734","position":2,"is_corresponding":false},{"id":372495,"name":"James D. Manton","orcid":"0000-0001-9260-3156","position":3,"is_corresponding":false},{"id":245883,"name":"Kevin M. Dean","orcid":"0000-0003-0839-2320","position":4,"is_corresponding":false},{"id":225169,"name":"Reto Fiolka","orcid":"0000-0002-4636-5000","position":5,"is_corresponding":false},{"id":353381,"name":"Bingying Chen","orcid":"0000-0003-3518-6290","position":0,"is_corresponding":true}],"reference_count":16,"raw_metadata":null,"created_at":"2026-07-18T21:43:11.119231Z","pmid":"33014569","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}