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Firefly-HR combines patterned stimulation, near-total internal reflection laser excitation through a prism located between the sample and a water-immersion objective, and concurrent imaging of three color channels. The microscope has efficient light collection, low fluorescent background, and a large field of view (0.24 x 1.2 mm @ 1000 frames/sec). We characterize optical crosstalk and demonstrate capabilities with three applications: (1) probing synaptically connected neuronal microcircuits, (2) examining the coupling between neuronal action potentials and calcium influx, and (3) studying the pharmacology of paced human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) via simultaneous recordings of voltage, calcium, and contraction.","is_dataset_classified":null,"base_score":3.8066624897703196,"endowment":3.8066624897703196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30800515","pmcid":"PMC6377900","openalex_id":"https://openalex.org/W2911435322","authors":[],"funders":[{"funder_name":"National Institute of Mental Health","grant_id":"R43MH112273","title":null},{"funder_name":"National Institute of Mental Health","grant_id":"R44MH112474","title":null}],"total_grants":2,"fwci":2.8583,"citation_percentile":0.91247303,"influential_citations":0,"citation_trend":[{"year":2019,"count":4},{"year":2020,"count":10},{"year":2021,"count":13},{"year":2022,"count":2},{"year":2023,"count":11},{"year":2024,"count":2},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"gold","license":"https://doi.org/10.1364/OA_License_v1#VOR-OA","oa_locations":[{"url":"https://doi.org/10.1364/boe.10.000789","host_type":"journal"},{"url":"https://doi.org/10.1364/boe.10.000789","host_type":"publisher"},{"url":"https://www.osapublishing.org/viewmedia.cfm?URI=boe-10-2-789&seq=0","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30800515","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC6377900","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6377900","host_type":"repository"}],"fields_of_study":["Photoreceptor and optogenetics research","Neuroscience and Neural Engineering","Neural dynamics and brain function"],"mesh_terms":[],"keywords":["Optogenetics","Calcium imaging","Optical recording","Calcium","Microscope","Voltage-sensitive dye","Microscopy","Materials science","Stimulation","Biophysics","Fluorescence","Optics","Neuroscience","Optoelectronics","Biology","Physics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T04:42:30.353467Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}