{"doi":"10.1292/jvms.16-0157","title":"Method for isolating pure bovine gonadotrophs from anterior pituitary using magnetic nanoparticles and anti-gonadotropin-releasing hormone receptor antibody","abstract":null,"journal":"Journal of Veterinary Medical Science","year":2016,"id":607461,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1559818,"name":"Ashrafun NAHAR","orcid":null,"position":1,"is_corresponding":false},{"id":1559819,"name":"Hiroya KADOKAWA","orcid":null,"position":2,"is_corresponding":false},{"id":1559817,"name":"Kiran PANDEY","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Method for isolating pure bovine gonadotrophs from anterior pituitary using magnetic nanoparticles and anti-gonadotropin-releasing hormone receptor antibody","abstract":"No methods are currently available for rapidly isolating gonadotrophs from the anterior pituitary (AP) in any species. We developed a method for preparing pure bovine gonadotrophs from a heterogeneous AP cell mixture by magnetic separation and our original antibody against the N terminus of bovine gonadotropin-releasing hormone receptor (GnRHR). A bovine AP cell mixture was incubated with the anti-GnRHR antibody, anti-dextran antibody-conjugated secondary antibody and dextran-coated magnetic nanoparticles for magnetic isolation. Approximately 5.2 × 106 cells were isolated per AP of Japanese Black heifers (26 months of age) and cultured, and confocal microscopy confirmed to be GnRHR- and luteinizing hormone-positive, corresponding to a purity of 100%. Approximately 44.5 µg of total protein was extracted from the pure gonadotrophs per AP.","is_dataset_classified":null,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27430292","pmcid":"PMC5138424","openalex_id":"https://openalex.org/W2461790828","authors":[],"funders":[],"total_grants":0,"fwci":1.7418,"citation_percentile":0.84932197,"influential_citations":0,"citation_trend":[{"year":2017,"count":2},{"year":2018,"count":1},{"year":2020,"count":1},{"year":2022,"count":2},{"year":2024,"count":1}],"oa_status":"gold","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.jstage.jst.go.jp/article/jvms/78/11/78_16-0157/_pdf","host_type":"journal"},{"url":"https://www.jstage.jst.go.jp/article/jvms/78/11/78_16-0157/_pdf","host_type":"publisher"},{"url":"https://doi.org/10.1292/jvms.16-0157","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27430292","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5138424","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5138424","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5138424?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Reproductive Physiology in Livestock","Growth Hormone and Insulin-like Growth Factors","Genetic and phenotypic traits in livestock"],"mesh_terms":["Animals","Antibodies","Cattle","Cells, Cultured","Female","Luteinizing Hormone","Pituitary Gland, Anterior","Receptors, LHRH","Immunomagnetic Separation","Gonadotrophs","Magnetite Nanoparticles"],"keywords":["GNRHR","Gonadotropic cell","Anterior pituitary","Gonadotropin-releasing hormone receptor","Gonadotropin-releasing hormone","Antibody","Luteinizing hormone","Chemistry","Receptor","Internal medicine","Endocrinology","Hormone","Biology","Biochemistry","Immunology","Medicine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T06:25:27.812475Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}