{"doi":"10.1292/jvms.15-0216","title":"Development of one-tube multiplex polymerase chain reaction (PCR) for detecting <i>Mycobacterium bovis</i>","abstract":null,"journal":"Journal of Veterinary Medical Science","year":2016,"id":631344,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1636071,"name":"Tan HAIMING","orcid":null,"position":1,"is_corresponding":false},{"id":1636074,"name":"Cai XIAOYAO","orcid":null,"position":2,"is_corresponding":false},{"id":1636075,"name":"Yuan WEIFENG","orcid":null,"position":3,"is_corresponding":false},{"id":1636077,"name":"Jia HONG","orcid":null,"position":4,"is_corresponding":false},{"id":1636079,"name":"Zhu HONGFEI","orcid":null,"position":5,"is_corresponding":false},{"id":1636069,"name":"Zhang QUAN","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Development of one-tube multiplex polymerase chain reaction (PCR) for detecting <i>Mycobacterium bovis</i>","abstract":"A multiplex PCR (m-PCR) with primers targeting the 16S rRNA, Rv3873 and a 12.7-kb fragment in the genomes of a Mycobacterium tuberculosis complex was designed for the differential diagnosis of M. tuberculosis, M. bovis, M. bovis BCG and non-tuberculosis Mycobacterium (NTM). The specificity of this assay was 100%, and the detection limit was 15 pg of genomic DNA. Of the 206 blinded clinical samples, the detection rate of M. bovis infection by m-PCR was lower than that of the interferon gamma (IFN-γ) release assay; however, the false-positive rate by the tuberculin skin test and false-negative samples in the IFN-γ release assay were reduced. Our findings indicated that our m-PCR method is a useful tool for complementation to differentiate M. bovis from M. tuberculosis and NTM species.","is_dataset_classified":null,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27534910","pmcid":"PMC5240767","openalex_id":"https://openalex.org/W2516442224","authors":[],"funders":[],"total_grants":0,"fwci":0.4289,"citation_percentile":0.66634673,"influential_citations":0,"citation_trend":[{"year":2019,"count":2},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":2}],"oa_status":"gold","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.jstage.jst.go.jp/article/jvms/78/12/78_15-0216/_pdf","host_type":"journal"},{"url":"https://www.jstage.jst.go.jp/article/jvms/78/12/78_15-0216/_pdf","host_type":"publisher"},{"url":"https://doi.org/10.1292/jvms.15-0216","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27534910","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5240767","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5240767","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5240767?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Tuberculosis Research and Epidemiology","Mycobacterium research and diagnosis","Diagnosis and treatment of tuberculosis"],"mesh_terms":["Animals","Cattle","DNA, Bacterial","Mycobacterium","Mycobacterium bovis","RNA, Ribosomal, 16S","Sensitivity and Specificity","Multiplex Polymerase Chain Reaction"],"keywords":["Mycobacterium bovis","Polymerase chain reaction","Multiplex polymerase chain reaction","Mycobacterium tuberculosis","Tuberculosis","Molecular biology","Biology","Microbiology","Real-time polymerase chain reaction","Multiplex","Mycobacterium","genomic DNA","Virology","DNA","Medicine","Gene","Genetics","Pathology"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T23:23:20.640223Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}