{"doi":"10.1261/rna.1129608","title":"3′-cyclic phosphorylation of U6 snRNA leads to recruitment of recycling factor p110 through LSm proteins","abstract":"<jats:p>Pre-mRNA splicing proceeds through assembly of the spliceosome complex, catalysis, and recycling. During each cycle the U4/U6.U5 tri-snRNP is disrupted and U4/U6 snRNA base-pairing unwound, releasing separate post-spliceosomal U4, U5, and U6 snRNPs, which have to be recycled to the splicing-competent tri-snRNP. Previous work implicated p110—the human ortholog of the yeast Prp24 protein—and the LSm2-8 proteins of the U6 snRNP in U4/U6 recycling. Here we show in vitro that these proteins bind synergistically to U6 snRNA: Both purified and recombinant LSm2-8 proteins are able to recruit p110 protein to U6 snRNA via interaction with the highly conserved C-terminal region of p110. Furthermore, the presence of a 2′,3′-cyclic phosphate enhances the affinity of U6 snRNA for the LSm2-8 proteins and inversely reduces La protein binding, suggesting a direct role of the 3′-terminal phosphorylation in RNP remodeling during U6 biogenesis.</jats:p>","journal":"RNA","year":2008,"id":595847,"datarank":0.5897738449086489,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"self_citation_contribution":0.5897738449086489,"citation_network_contribution":0.0,"self_endowment_contribution":0.5897738449086489,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":50,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":17851,"name":"Jan Medenbach","orcid":"0000-0003-0749-7788","position":1,"is_corresponding":false},{"id":178599,"name":"Reinhard Lührmann","orcid":"0000-0003-3253-7518","position":2,"is_corresponding":false},{"id":810799,"name":"Christian Kambach","orcid":null,"position":3,"is_corresponding":false},{"id":179894,"name":"Albrecht Bindereif","orcid":null,"position":4,"is_corresponding":false},{"id":1525944,"name":"Konstantin Licht","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"3′-cyclic phosphorylation of U6 snRNA leads to recruitment of recycling factor p110 through LSm proteins","abstract":"<jats:p>Pre-mRNA splicing proceeds through assembly of the spliceosome complex, catalysis, and recycling. During each cycle the U4/U6.U5 tri-snRNP is disrupted and U4/U6 snRNA base-pairing unwound, releasing separate post-spliceosomal U4, U5, and U6 snRNPs, which have to be recycled to the splicing-competent tri-snRNP. Previous work implicated p110—the human ortholog of the yeast Prp24 protein—and the LSm2-8 proteins of the U6 snRNP in U4/U6 recycling. Here we show in vitro that these proteins bind synergistically to U6 snRNA: Both purified and recombinant LSm2-8 proteins are able to recruit p110 protein to U6 snRNA via interaction with the highly conserved C-terminal region of p110. Furthermore, the presence of a 2′,3′-cyclic phosphate enhances the affinity of U6 snRNA for the LSm2-8 proteins and inversely reduces La protein binding, suggesting a direct role of the 3′-terminal phosphorylation in RNP remodeling during U6 biogenesis.</jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18567812","pmcid":"PMC2491463","openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":"other-oa","oa_locations":[{"url":"http://rnajournal.cshlp.org/content/14/8/1532.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1261/rna.1129608","host_type":"publisher"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2491463","host_type":"repository"},{"url":"http://hdl.handle.net/11858/00-001M-0000-0012-DB28-1","host_type":"repository"}],"fields_of_study":[],"mesh_terms":["Hela Cells","Humans","RNA-Binding Proteins","Ribonucleoproteins","Ribonucleoprotein, U4-U6 Small Nuclear","Nuclear Proteins","RNA, Small Nuclear","Antigens, Neoplasm","Autoantigens","RNA Splicing","Base Sequence","Molecular Sequence Data","SS-B Antigen"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T17:57:53.445348Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}