{"doi":"10.1261/rna.079959.124","title":"Challenges to mapping and defining m<sup>6</sup>A function in viral RNA","abstract":"Viral RNA molecules contain multiple layers of regulatory information. This includes features beyond the primary sequence, such as RNA structures and RNA modifications, including N6-methyladenosine (m 6 A). Many recent studies have identified the presence and location of m 6 A in viral RNA and have found diverse regulatory roles for this modification during viral infection. However, to date, viral m 6 A mapping strategies have limitations that prevent a complete understanding of the function of m 6 A on individual viral RNA molecules. While m 6 A sites have been profiled on bulk RNA from many viruses, the resulting m 6 A maps of viral RNAs described to date present a composite picture of m 6 A across viral RNA molecules in the infected cell. Thus, for most viruses, it is unknown if unique viral m 6 A profiles exist throughout infection, nor if they regulate specific viral life cycle stages. Here, we describe several challenges to defining the function of m 6 A in viral RNA molecules and provide a framework for future studies to help in the understanding of how m 6 A regulates viral infection.","journal":"RNA","year":2024,"id":440034,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9459,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":302508,"name":"Matthew G. Thompson","orcid":"0000-0003-3813-9254","position":1,"is_corresponding":false},{"id":229072,"name":"Stacy M. Horner","orcid":"0000-0002-9351-7409","position":0,"is_corresponding":true}],"reference_count":89,"raw_metadata":null,"created_at":"2026-07-19T02:00:57.233748Z","pmid":"38531643","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}