{"doi":"10.1261/rna.078916.121","title":"SnoRNA guide activities: real and ambiguous","abstract":"<jats:p>\n                    In eukaryotes, rRNAs and spliceosomal snRNAs are heavily modified post-transcriptionally. Pseudouridylation and 2′-\n                    <jats:italic>O</jats:italic>\n                    -methylation are the most abundant types of RNA modifications. They are mediated by modification guide RNAs, also known as small nucleolar (sno)RNAs and small Cajal body-specific (sca)RNAs. We used yeast and vertebrate cells to test guide activities predicted for a number of snoRNAs, based on their regions of complementarity with rRNAs. We showed that human SNORA24 is a genuine guide RNA for 18S-Ψ609, despite some noncanonical base-pairing with its target. At the same time, we found quite a few snoRNAs that have the ability to base-pair with rRNAs and can induce predicted modifications in artificial substrate RNAs, but do not modify the same target sequence within endogenous rRNA molecules. Furthermore, certain fragments of rRNAs can be modified by the endogenous yeast modification machinery when inserted into an artificial backbone RNA, even though the same sequences are not modified in endogenous yeast rRNAs. In\n                    <jats:italic>Xenopus</jats:italic>\n                    cells, a guide RNA generated from scaRNA, but not from snoRNA, could induce an additional pseudouridylation of U2 snRNA at position 60; both guide RNAs were equally active on a U2 snRNA-specific substrate in yeast cells. Thus, post-transcriptional modification of functionally important RNAs, such as rRNAs and snRNAs, is highly regulated and more complex than simply strong base-pairing between a guide RNA and substrate RNA. We discuss possible regulatory roles for these unexpected modifications.\n                  </jats:p>","journal":"RNA","year":2021,"id":33984,"datarank":0.6095605407456629,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.2248181371264323,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.2248181371264323,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":12,"citers_with_citation_signal":9,"citers_with_endowment":9,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":172154,"name":"Gaëlle J.S. Talross","orcid":null,"position":1,"is_corresponding":false},{"id":172156,"name":"Joseph G. Gall","orcid":"0000-0001-9664-6559","position":2,"is_corresponding":false},{"id":172152,"name":"Svetlana Deryusheva","orcid":"0000-0002-6127-1889","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34385348","pmcid":"PMC8522698","openalex_id":"https://openalex.org/W3190591694","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM033397","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01GM033397-05","title":"THE ORGANIZATION OF ANIMAL CELL NUCLEI"}],"total_grants":2,"fwci":0.7327,"citation_percentile":0.69180962,"influential_citations":0,"citation_trend":[{"year":2022,"count":5},{"year":2023,"count":2},{"year":2024,"count":2},{"year":2025,"count":3}],"oa_status":"bronze","license":"CC BY","oa_locations":[{"url":"http://rnajournal.cshlp.org/content/27/11/1363.full.pdf","host_type":"journal"},{"url":"https://doi.org/10.1261/rna.078916.121","host_type":"BRONZE"},{"url":"http://rnajournal.cshlp.org/content/27/11/1363.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1261/rna.078916.121","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34385348","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC8522698","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8522698","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC8522698","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC8522698?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1101/2021.07.15.452550","host_type":""},{"url":"https://europepmc.org/articles/pmc8522698?pdf=render","host_type":""},{"url":"http://dx.doi.org/10.1261/rna.078916.121","host_type":""},{"url":"https://dx.doi.org/10.1261/rna.078916.121","host_type":""},{"url":"https://dx.doi.org/10.1101/2021.07.15.452550","host_type":""}],"fields_of_study":["RNA modifications and cancer","Cancer-related gene regulation","RNA and protein synthesis mechanisms","Medicine","Biology","0301 basic medicine","03 medical and health sciences","Base Sequence","Coiled Bodies","Humans","Methylation","Pseudouridine","RNA Processing, Post-Transcriptional","RNA, Ribosomal","RNA, Small Nucleolar","Spliceosomes","RNA, Guide, CRISPR-Cas Systems"],"mesh_terms":["RNA, Guide, CRISPR-Cas Systems","Base Sequence","Humans","Methylation","Pseudouridine","RNA Processing, Post-Transcriptional","RNA, Ribosomal","Spliceosomes","RNA, Guide, Kinetoplastida","RNA, Small Nucleolar","Coiled Bodies"],"keywords":["Small nucleolar RNA","Biology","Small nuclear RNA","RNA","Guide RNA","Pseudouridine","Non-coding RNA","Base pair","Ribosomal RNA","Genetics","Computational biology","Transfer RNA","Cell biology","Gene","Genome","Snorna","2′-O-methylation","Pseudouridylation","Modification Guide Rna Activity","Pus7p","Base Sequence","RNA, Ribosomal","Spliceosomes","Humans","RNA, Small Nucleolar","Coiled Bodies","RNA Processing, Post-Transcriptional","RNA, Guide, CRISPR-Cas Systems","Methylation","Article"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T17:21:56.882486Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}