{"doi":"10.1248/bpb.b13-00132","title":"<i>In Vivo</i> Pharmacokinetics and Pharmacodynamics of Positional Isomers of Mono-PEGylated Recombinant Human Granulocyte Colony Stimulating Factor in Rats","abstract":null,"journal":"Biological and Pharmaceutical Bulletin","year":2013,"id":617563,"datarank":0.6904497656285552,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.3450620016794483,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.3450620016794483,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":9,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":625040,"name":"Kang Choon Lee","orcid":null,"position":1,"is_corresponding":false},{"id":1592716,"name":"Jung Seok Kang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"<i>In Vivo</i> Pharmacokinetics and Pharmacodynamics of Positional Isomers of Mono-PEGylated Recombinant Human Granulocyte Colony Stimulating Factor in Rats","abstract":"In this study, the pharmacokinetic and pharmacodynamic properties of Lys(35), Met(N-terminal), and Lys(17)-mono-PEGylated recombinant human granulocyte colony stimulating factor (rhG-CSF) positional isomers were evaluated in rats. The in vitro biological activities of Lys(35), Met(N-terminal), and Lys(17)-mono-PEGylated rhG-CSF were determined by examining NFS-60 cell proliferation. Plasma concentrations of rhG-CSF and white blood cell (WBC) counts and absolute neutrophil conunt (ANC) were measured and pharmacokinetic and pharmacodynamic properties were determined after a single subcutaneous administration of the Lys(35), Met(N-terminal), or Lys(17) isomers at 0.1 mg/kg in rats. The in vitro biological activities of Lys(35), Met(N-terminal), and Lys(17)-mono-PEGylated rhG-CSF individual positional isomers were 20.1%, 37.4%, and 15.3%, respectively, that of rhG-CSF. However, all three mono-PEGylated rhG-CSF isomers had a greater blood half-life (T1/2) and in vivo efficacy as determined by WBC counts and ANC than rhG-CSF, but no significant difference was observed between the three isomers. In conclusion, Lys(35), Met(N-terminal), and Lys(17)-mono-PEGylated rhG-CSF individual positional isomers exhibit an enhanced the in vivo pharmacokinetics and pharmacodynamics. Furthermore, three isomers have comparable in vivo pharmacokinetic and pharmacodynamic properties, but their in vitro biological activities are PEGylation site dependent.","is_dataset_classified":null,"base_score":2.302585092994046,"endowment":2.302585092994046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23811564","pmcid":null,"openalex_id":"https://openalex.org/W1998587742","authors":[],"funders":[],"total_grants":0,"fwci":0.1348,"citation_percentile":0.53799717,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2018,"count":1},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":2}],"oa_status":"gold","license":null,"oa_locations":[{"url":"https://www.jstage.jst.go.jp/article/bpb/36/7/36_b13-00132/_pdf","host_type":"journal"},{"url":"https://www.jstage.jst.go.jp/article/bpb/36/7/36_b13-00132/_pdf","host_type":"publisher"},{"url":"https://doi.org/10.1248/bpb.b13-00132","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23811564","host_type":"repository"}],"fields_of_study":["Immune Response and Inflammation","Neutropenia and Cancer Infections","Blood disorders and treatments"],"mesh_terms":["Animals","Binding Sites","Humans","Injections, Subcutaneous","Isomerism","Leukocyte Count","Lysine","Male","Methionine","Models, Molecular","Neutrophils","Polyethylene Glycols","Structure-Activity Relationship","Linear Models","Granulocyte Colony-Stimulating Factor","Rats, Sprague-Dawley","Rats"],"keywords":["Pharmacokinetics","PEGylation","In vivo","Pharmacodynamics","Pharmacology","In vitro","Granulocyte colony-stimulating factor","Chemistry","Medicine","Biochemistry","Biology","Internal medicine","Chemotherapy"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T02:02:01.877865Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}