{"doi":"10.1242/jcs.261097","title":"UMAD1 contributes to ESCRT-III dynamic subunit turnover during cytokinetic abscission","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>Abscission is the final stage of cytokinesis whereby the midbody, a thin intercellular bridge, is resolved to separate the daughter cells. Cytokinetic abscission is mediated by the endosomal sorting complex required for transport (ESCRT), a conserved membrane remodelling machinery. The midbody organiser CEP55 recruits early acting ESCRT factors such as ESCRT-I and ALIX (also known as PDCD6IP), which subsequently initiate the formation of ESCRT-III polymers that sever the midbody. We now identify UMAD1 as an ESCRT-I subunit that facilitates abscission. UMAD1 selectively associates with VPS37C and VPS37B, supporting the formation of cytokinesis-specific ESCRT-I assemblies. TSG101 recruits UMAD1 to the site of midbody abscission, to stabilise the CEP55–ESCRT-I interaction. We further demonstrate that the UMAD1–ESCRT-I interaction facilitates the final step of cytokinesis. Paradoxically, UMAD1 and ALIX co-depletion has synergistic effects on abscission, whereas ESCRT-III recruitment to the midbody is not inhibited. Importantly, we find that both UMAD1 and ALIX are required for the dynamic exchange of ESCRT-III subunits at the midbody. Therefore, UMAD1 reveals a key functional connection between ESCRT-I and ESCRT-III that is required for cytokinesis.</jats:p>","journal":"Journal of Cell Science","year":2023,"id":42232,"datarank":0.2523293965845767,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.010913709719461623,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.010913709719461623,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":2,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":202426,"name":"Edward J. Scourfield","orcid":null,"position":1,"is_corresponding":false},{"id":202427,"name":"Leandro N. Ventimiglia","orcid":"0000-0002-2872-1497","position":2,"is_corresponding":false},{"id":54725,"name":"Xiaoping Yang","orcid":"0000-0002-6811-4915","position":3,"is_corresponding":false},{"id":202428,"name":"Steven Lynham","orcid":"0000-0003-3756-6430","position":4,"is_corresponding":false},{"id":201620,"name":"Monica Agromayor","orcid":"0000-0001-6477-1750","position":5,"is_corresponding":false},{"id":201621,"name":"Juan Martin-Serrano","orcid":"0000-0002-8183-3914","position":6,"is_corresponding":false},{"id":202425,"name":"James Glover","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37439191","pmcid":"PMC10445733","openalex_id":"https://openalex.org/W4384120486","authors":[],"funders":[{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BB/N000501/1","title":"Understanding the role of the ESCRT-associated protein MITD1 in linking cytoskeleton and membrane remodelling"},{"funder_name":"King's Health Partners","grant_id":"R151001","title":null},{"funder_name":"Wellcome Trust","grant_id":"WT102871MA","title":null},{"funder_name":"Medical Research Council","grant_id":"MR/K50130X/1","title":"DTG 2013 &amp; 2014"},{"funder_name":"Medical Research Council","grant_id":"MR/N013700/1","title":"DTP Bid Led by King's College London"},{"funder_name":"Wellcome Trust","grant_id":"102871","title":"Exploring the final events in eukaryotic cell division."},{"funder_name":"King&apos;s College London","grant_id":"","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null}],"total_grants":9,"fwci":0.5774,"citation_percentile":0.66975843,"influential_citations":0,"citation_trend":[{"year":2023,"count":1},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.261097/3038512/jcs261097.pdf","host_type":"journal"},{"url":"https://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.261097/3038512/jcs261097.pdf","host_type":"HYBRID"},{"url":"https://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.261097/3038512/jcs261097.pdf","host_type":"publisher"},{"url":"https://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.261097/3102586/jcs261097.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/jcs.261097","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/37439191","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10445733","host_type":"repository"},{"url":"https://kclpure.kcl.ac.uk/portal/en/publications/707a7529-325e-4879-871a-d47c01216ea6","host_type":""},{"url":"https://www.scopus.com/pages/publications/85167843736","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC10445733","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1242/jcs.261097","host_type":""},{"url":"https://doi.org/https://doi.org/10.1242/JCS.261097","host_type":""}],"fields_of_study":["Cellular transport and secretion","Erythrocyte Function and Pathophysiology","Calcium signaling and nucleotide metabolism","Medicine","Biology","Cytokinesis","Endosomal Sorting Complexes Required for Transport","Cell Cycle Proteins"],"mesh_terms":["Cell Cycle Proteins","Cytokinesis","Endosomal Sorting Complexes Required for Transport"],"keywords":["Midbody","ESCRT","Cytokinesis","Abscission","Biology","Cell biology","TSG101","Endosome","Cell division","Intracellular","Biochemistry","Genetics","Cell","Membrane Remodelling","Endosomal Sorting Complexes Required for Transport","500","Cell Cycle Proteins","540","Research Article"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pxd"},{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-14T00:13:31.090397Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}