{"doi":"10.1242/jcs.259626","title":"A CDK activity buffer ensures mitotic completion","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>The eukaryotic cell cycle is driven by the activity of cyclin-dependent kinases (CDKs). CDK activity rises over 50-fold during the cell cycle, from a low level in G1 to a high level in mitosis. However, it is not known whether the entire range of CDK activity is necessary for cell cycle progression, or whether cells can tolerate a reduction in CDK activity level. Here, in fission yeast, we show that sublethal CDK inhibition lengthens the time cells spend in mitosis but does not cause misordering of mitotic events. Maximum attainable CDK activity exceeds the amount necessary for mitosis, and thus forms a CDK activity buffer between sufficient and maximal possible CDK activities. This CDK activity buffer is needed for mitotic completion when CDK activity is compromised, and CDK inhibition only becomes lethal to cells when this buffer is exhausted. Finally, we explore what factors influence this CDK activity buffer, and find that it is influenced by CDK-counteracting phosphatases. Therefore, maximum attainable CDK activity is not necessary for mitosis but provides robustness to CDK activity reduction to ensure mitotic completion.</jats:p>","journal":"Journal of Cell Science","year":2022,"id":42401,"datarank":0.2829566857600593,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.04154099889494422,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.04154099889494422,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":2,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":91486,"name":"James O. Patterson","orcid":"0000-0003-1993-4500","position":1,"is_corresponding":false},{"id":203023,"name":"Theresa U. Zeisner","orcid":"0000-0002-2898-289X","position":2,"is_corresponding":false},{"id":203024,"name":"Paul Nurse","orcid":"0000-0002-9244-7787","position":3,"is_corresponding":false},{"id":203022,"name":"Souradeep Basu","orcid":"0000-0003-4448-8688","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35726599","pmcid":"PMC9270952","openalex_id":"https://openalex.org/W4283217815","authors":[],"funders":[{"funder_name":"Cancer Research UK","grant_id":"FC01121","title":null},{"funder_name":"Wellcome Trust","grant_id":"093917","title":"Networks for global cellular controls in fission yeast"},{"funder_name":"Wellcome Trust","grant_id":"214183","title":"Global Cellular Controls in Fission Yeast"},{"funder_name":"The Francis Crick Institute","grant_id":"CC2003","title":null},{"funder_name":"Wellcome Trust","grant_id":"FC001121","title":null},{"funder_name":"The Francis Crick Institute","grant_id":"10121","title":null},{"funder_name":"Wellcome Trust","grant_id":"214183/Z/18/Z","title":null},{"funder_name":"Lord Leonard and Lady Estelle Wolfson Foundation","grant_id":"","title":null},{"funder_name":"Boehringer Ingelheim Fonds","grant_id":"","title":null},{"funder_name":"Francis Crick Institute","grant_id":"","title":null},{"funder_name":"Woosnam Foundation","grant_id":"","title":null},{"funder_name":"Francis Crick Institute","grant_id":"","title":null},{"funder_name":"Lord Leonard and Lady Estelle Wolfson Foundation","grant_id":"","title":null},{"funder_name":"Woosnam Foundation","grant_id":"","title":null},{"funder_name":"Boehringer Ingelheim Fonds","grant_id":"","title":null}],"total_grants":15,"fwci":0.4657,"citation_percentile":0.56378776,"influential_citations":0,"citation_trend":[{"year":2022,"count":1},{"year":2025,"count":3}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1242/jcs.259626","host_type":"journal"},{"url":"https://doi.org/10.1242/jcs.259626","host_type":"HYBRID"},{"url":"https://doi.org/10.1242/jcs.259626","host_type":"publisher"},{"url":"https://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.259626/3489457/jcs259626.pdf","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/35726599","host_type":"repository"},{"url":"https://figshare.com/articles/journal_contribution/A_CDK_activity_buffer_ensures_mitotic_completion_/20145458","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9270952","host_type":"repository"},{"url":"https://doi.org/10.25418/crick.20145458.v1","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9270952","host_type":"Europe_PMC"},{"url":"https://dx.doi.org/10.25418/crick.20145458.v1","host_type":""},{"url":"https://dx.doi.org/10.25418/crick.20145458","host_type":""},{"url":"http://dx.doi.org/10.1242/jcs.259626","host_type":""}],"fields_of_study":["Microtubule and mitosis dynamics","Fungal and yeast genetics research","Genomics and Chromatin Dynamics","Medicine","Biology","0301 basic medicine","0303 health sciences","03 medical and health sciences","Cell Cycle Proteins","Cyclin-Dependent Kinases","Mitosis","Phosphorylation","Schizosaccharomyces","Schizosaccharomyces pombe Proteins"],"mesh_terms":["Mitosis","Phosphorylation","Schizosaccharomyces","Cell Cycle Proteins","Cyclin-Dependent Kinases","Schizosaccharomyces pombe Proteins"],"keywords":["Cyclin-dependent kinase","Mitosis","Biology","Cell biology","Polo-like kinase","Mitotic exit","Cell cycle","CDK inhibitor","Cell","Biochemistry","Anaphase","Schizosaccharomyces pombe","kinase activity","Cdk1","Model organisms","Chemical Biology &amp; High Throughput","Short Report","Cell Cycle Proteins","Biochemistry &amp; Proteomics","Cyclin-Dependent Kinases","Schizosaccharomyces","Cell Cycle &amp; Chromosomes","Synthetic Biology","Schizosaccharomyces pombe Proteins","Phosphorylation","Genetics &amp; Genomics","Computational &amp; Systems Biology"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-14T02:47:07.121702Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}