{"doi":"10.1242/jcs.259010","title":"<i>In vivo</i> imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip","abstract":"Flagellar assembly depends on intraflagellar transport (IFT), a bidirectional motility of protein carriers, the IFT trains. The trains are periodic assemblies of IFT-A and IFT-B subcomplexes and the motors kinesin-2 and IFT dynein. At the tip, anterograde trains are remodeled for retrograde IFT, a process that in Chlamydomonas involves kinesin-2 release and train fragmentation. However, the degree of train disassembly at the tip remains unknown. Here, we performed two-color imaging of fluorescent protein-tagged IFT components, which indicates that IFT-A and IFT-B proteins from a given anterograde train usually return in the same set of retrograde trains. Similarly, concurrent turnaround was typical for IFT-B proteins and the IFT dynein subunit D1bLIC-GFP but severance was observed as well. Our data support a simple model of IFT turnaround, in which IFT-A, IFT-B and IFT dynein typically remain associated at the tip and segments of the anterograde trains convert directly into retrograde trains. Continuous association of IFT-A, IFT-B and IFT dynein during tip remodeling could balance protein entry and exit, preventing the build-up of IFT material in flagella.","journal":"Journal of Cell Science","year":2021,"id":168759,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9522,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":698843,"name":"Betlehem Mekonnen","orcid":null,"position":1,"is_corresponding":false},{"id":88244,"name":"Ilaria Mengoni","orcid":null,"position":2,"is_corresponding":false},{"id":320832,"name":"Peiwei Liu","orcid":"0000-0001-5597-2200","position":3,"is_corresponding":false},{"id":698332,"name":"Mareike A. Jordan","orcid":"0000-0001-6248-8863","position":4,"is_corresponding":false},{"id":698333,"name":"Dennis R. Diener","orcid":"0000-0001-8564-2907","position":5,"is_corresponding":false},{"id":698334,"name":"Gaia Pigino","orcid":"0000-0002-2295-9568","position":6,"is_corresponding":false},{"id":309347,"name":"Karl F. Lechtreck","orcid":"0000-0002-6219-6470","position":7,"is_corresponding":false},{"id":309344,"name":"Jenna L. Wingfield","orcid":"0000-0002-9522-4183","position":0,"is_corresponding":true}],"reference_count":72,"raw_metadata":null,"created_at":"2026-07-18T23:46:06.853307Z","pmid":"34415027","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}