{"doi":"10.1242/jcs.243303","title":"CENP-B creates alternative epigenetic chromatin states permissive for CENP-A or heterochromatin assembly","abstract":"ABSTRACT CENP-B binds to CENP-B boxes on centromeric satellite DNAs (known as alphoid DNA in humans). CENP-B maintains kinetochore function through interactions with CENP-A nucleosomes and CENP-C. CENP-B binding to transfected alphoid DNA can induce de novo CENP-A assembly, functional centromere and kinetochore formation, and subsequent human artificial chromosome (HAC) formation. Furthermore, CENP-B also facilitates H3K9 (histone H3 lysine 9) trimethylation on alphoid DNA, mediated by Suv39h1, at ectopic alphoid DNA integration sites. Excessive heterochromatin invasion into centromere chromatin suppresses CENP-A assembly. It is unclear how CENP-B controls such different chromatin states. Here, we show that the CENP-B acidic domain recruits histone chaperones and many chromatin modifiers, including the H3K36 methylase ASH1L, as well as the heterochromatin components Suv39h1 and HP1 (HP1α, β and γ, also known as CBX5, CBX1 and CBX3, respectively). ASH1L facilitates the formation of open chromatin competent for CENP-A assembly on alphoid DNA. These results indicate that CENP-B is a nexus for histone modifiers that alternatively promote or suppress CENP-A assembly by mutually exclusive mechanisms. Besides the DNA-binding domain, the CENP-B acidic domain also facilitates CENP-A assembly de novo on transfected alphoid DNA. CENP-B therefore balances CENP-A assembly and heterochromatin formation on satellite DNA.","journal":"Journal of Cell Science","year":2020,"id":65658,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":56,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9504,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":347644,"name":"Jun‐ichirou Ohzeki","orcid":"0009-0002-3831-4489","position":1,"is_corresponding":false},{"id":349402,"name":"Nobuaki Shono","orcid":null,"position":2,"is_corresponding":false},{"id":347645,"name":"Kazuto Kugou","orcid":"0000-0002-7690-1249","position":3,"is_corresponding":false},{"id":349403,"name":"Koei Okazaki","orcid":null,"position":4,"is_corresponding":false},{"id":165968,"name":"Takahiro Nagase","orcid":null,"position":5,"is_corresponding":false},{"id":347646,"name":"Hisashi Yamakawa","orcid":"0000-0002-1360-8328","position":6,"is_corresponding":false},{"id":330899,"name":"Natalay Kouprina","orcid":"0000-0001-8690-5625","position":7,"is_corresponding":false},{"id":330900,"name":"Vladimir Larionov","orcid":"0000-0002-7996-4894","position":8,"is_corresponding":false},{"id":112566,"name":"Hiroshi Kimura","orcid":"0000-0003-0854-083X","position":9,"is_corresponding":false},{"id":347647,"name":"William C. Earnshaw","orcid":"0000-0002-7191-0621","position":10,"is_corresponding":false},{"id":347648,"name":"Hiroshi Masumoto","orcid":"0000-0001-6960-4417","position":11,"is_corresponding":false},{"id":347643,"name":"Koichiro Otake","orcid":"0000-0002-4021-2572","position":0,"is_corresponding":true}],"reference_count":82,"raw_metadata":null,"created_at":"2026-07-18T21:13:43.728351Z","pmid":"32661090","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}